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核农学报  2014 

不同氮浓度下茶树氮合成关键酶基因的表达分析

DOI: 10.11869/j.issn.100-8551.2014.06.0985, PP. 985-989

Keywords: 缺氮,基因,克隆,荧光定量PCR

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Abstract:

本研究以10月龄扦插九龙袍茶苗[Camelliasinensis(L.)O.Kuntzecv.Jiulongpao]为试验材料,通过沙培试验,设4个N浓度(0、60、600、6000mmol·L-1),每周3次,处理后21周,克隆得到茶叶中氮合成转化关键酶基因:谷氨酸脱氢酶(GDH)、谷氨酰胺合成酶(GS)、谷氨酰胺α-酮戊二酸氨基转移酶(GOGAT)的保守区,在GenBank登录号分别:JN602371、JN602372、JN602373;进行荧光定量PCR检测,发现缺氮下GS、GOGAT基因的表达增强;GDH基因表达显著下降。通过回归分析,GS基因表达与叶片氮含量呈负相关,而GDH与叶片氮含量呈正相关。

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