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核农学报  2014 

莲草直胸跳甲Hsp70基因克隆及高温表达分析

DOI: 10.11869/j.issn.100-8551.2014.06.0970, PP. 970-977

Keywords: 莲草直胸跳甲,Hsp70,高温胁迫,实时定量PCR

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Abstract:

为揭示热激蛋白(heatshockproteins,Hsps)基因和莲草直胸跳甲Agasicleshygrophila温度胁迫适应性的关系,克隆了莲草直胸跳甲Hsp70基因序列,采用实时荧光定量PCR的方法检测了25~42.5℃莲草直胸跳甲成虫体内Hsp70的表达情况。结果表明:所克隆序列含有Hsp70家族的典型基序和位点,并与其它昆虫Hsp70的序列相似性很高,其中与马铃薯甲虫的相似性达到94%,表明所克隆序列为Hsp70靶基因,并对序列进行了系统进化分析;莲草直胸跳甲Hsp70的诱导起始温度为32.5℃(Ton),诱导最高温度为37.5℃(Tmax);Hsp70的Tmax能代表莲草直胸跳甲的耐受极限温度,是揭示物种耐受性的很好指标。本研究结果表明Hsp70在莲草直胸跳甲的高温适应性中可能起重要作用。

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