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核农学报  2014 

猕猴桃溃疡病病原菌分子鉴定与抗性材料初选

DOI: 10.11869/j.issn.100-8551.2014.07.181, PP. 1181-1187

Keywords: 猕猴桃,溃疡病,分子鉴定,抗性,筛选

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Abstract:

为明确我国南方猕猴桃细菌性溃疡病频发区其致病菌的种类与特征,初选出抗性种质材料,以猕猴桃主栽品种红阳、徐香和金丰的溃疡病感病枝条为材料,采用KB培养基、平板划线和梯度稀释法分离病原菌,利用基于细菌16S-23SrDNA内转录间隔区序列设计的特异性鉴别引物,对分离得到的6个代表菌株进行PCR扩增和测序,获得了大小约为280bp的特异性片段,其测序结果与登录号为D86357和AY342165的Pseudomonassyringaepv.Actinidia的序列完全一致。采用离体叶片注射接种、离体枝条针刺接种和活体枝杆针刺接种3种方法鉴定了18份种质材料的抗性。分析结果表明,红阳等中华猕猴桃最感,布鲁诺实生优株13-3、13-4等最抗,美味猕猴桃海沃德居中。本试验所分离的菌株均为丁香假单胞杆菌猕猴桃致病变种(Pseudomonassyringaepv.Actinidia),筛选出2份高抗溃疡病的种质材料。为进一步开展猕猴档细菌性溃疡病防治研究奠定基础。

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