全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

水稻抽穗期QTLqHD3的精细定位

DOI: 10.11869/j.issn.100-8551.2015.10.1845, PP. 1845-1851

Keywords: 水稻,单片段代换系,抽穗期,精细定位

Full-Text   Cite this paper   Add to My Lib

Abstract:

为鉴定和定位水稻抽穗期基因(QTLs),以受体相同而供体不同的2个单片段代换系为材料,使用单片段代换系之间杂交发展的衍生分离群体(F3和F4),对水稻抽穗期QTL-qHD3展开遗传和精细定位研究.在作图群体中,早抽穗和晚抽穗植株数符合3:1的分离比,早抽穗表现为显性.应用S1-27和D19随机群体(266和879个单株)将qHD3初步定位于第3染色体短臂SSR标记RM14314和RM569之间,遗传距离分别为0.3~0.4和2.0~2.2cM.应用D19分离群体的6680个单株对qHD3进行精细定位,经重组分析,最终将qHD3定位在RM14314和RM14320之间约62.4kb的染色体区间,该染色体区间共发现7个可表达的基因克隆,其中OsMADS50可能与qHD3等位.该研究结果为qHD3基因克隆及功能分析提供了理论依据.

References

[1]  Eshed Y, Zamir D. An introgression line population of lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL[J]. Genetics, 1995, 141(3): 1147-1162
[2]  Yamamoto T, Lin H, Sasaki T, Yano M. Identification of heading date quantitative trait locus Hd6, and characterization of its epistatic interaction with Hd2 in rice using advanced backcross progeny[J]. Genetics, 2000, 154(2): 885-891
[3]  Lin H X, Yamamoto T, Sasaki T, Yano M. Characterization and detection of epistatic interactions of 3 QTLs, Hd1, Hd2, and Hd3, controlling heading date in rice using nearly isogenic lines[J]. Theoretical and Applied Genetics, 2000, 101(7): 1021-1028
[4]  Lin H X, Ashikari M, Yamanouchi U, Sasaki T, Yano M. Identification and characterization of a quantitative trait locus Hd9, controlling heading date in rice[J]. Breeding Science, 2002, 52(1): 35-41
[5]  Monna L, Lin H X, Kojima S, Sasaki T, Yano M. Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice[J]. Theoretical and Applied Genetics, 2002, 104(5): 772-778
[6]  Lin H G, Liang Z W, Sasaki T, Yano M. Fine mapping and characterization of quantitative trait loci Hd4 and Hd5 controlling heading date in rice[J]. Breeding Science, 2003, 53(1): 51-59
[7]  Takahashi Y, Shomura A, Sasaki T, Yano M. Hd6 a rice quantitative trait locus involved in photoperiod sensitivity, encodes the alpha subunit of protein kinase CK2[J]. Proceedings of the National Academy of Sciences, 2001, 98(14): 7922-7927
[8]  Kojima S, Takahashi Y, Kobayashi Y, Monna L, Sasaki T, Araki T, Yano M. Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day condition[J]. Plant and Cell Physiology, 2002, 43(10): 1096-1105
[9]  Wu W X, Zheng X M, Lu G W, Zhong Z Z, Gao H, Chen L P, Wu C Y, Wang H J, Wang Q, Zhou K N, Wang J L, Wu F Q, Zhang X, Guo X P, Cheng Z J, Lei C L, Lin Q B, Jiang L, Wang H Y, Song G, Wan J M. Association of functional nucleotide polymorphisms at DTH2 with the northward expansion of rice cultivation in Asia[J]. Proceedings of the National Academy of Sciences, 2013, 110 (8): 2775-2780
[10]  Hori K, Ogiso-Tanaka E, Matsubara K, Yamanouchi U, Ebana K, Yano M. Hd16, a gene for casein kinase I, is involved in the control of rice flowering time by modulating the day-length response[J]. Plant Journal, 2013, 76(1): 36-46
[11]  Matsubara K, Ogiso-Tanaka E, Hori K, Ebana K, Ando T, Yano M. Natural variation in Hd17, a homolog of Arabidopsis ELF3 that is involved in rice photoperiodic flowering[J]. Plant and Cell Physiology , 2012, 53(4): 709-716
[12]  Xue W Y, Xing Y Z, Weng X Y, Zhao Y, Tang W J, Wang L, Zhou H J, Yu S B, Xu C G, Li X H, Zhang Q F. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nature Genetics, 2008, 40(6): 761-767
[13]  Koo B H, Yoo S C, Park J W, Kwon C T, Lee B D, An G, Zhang Z, Li J, Li Z, Paek N C. Natural variation in OsPRR37 regulates heading date and contributes to rice cultivation at a wide range of latitudes[J]. Molecular Plant, 2013, 6 (6): 1877-1888
[14]  Yan W H, Wang P, Chen H X, Zhou H J, Li Q P, Wang C R, Ding Z H, Zhang Y S, Yu S B, Xing Y Z, Zhang Q F. A Major QTL, Ghd8, plays pleiotropic roles in regulating grain productivity, plant height, and heading date in rice[J]. Molecular Plant, 2011, 4(2): 319-330
[15]  Doi K, Izawa T, Fuse T, Yamanouchi U, Kubo T, Shimatani Z, Yano M, Yoshimura A. Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1[J]. Genes & Development, 2004, 18(8): 926-936
[16]  Zhang G Q, Zeng R Z, Zhang Z M, Ding X H, Li W T, Liu G M, He F H, Tulukdar A, Huang C F, Xi Z Y, Qin L J, Shi J Q, Zhao F M, Feng M J, Shan Z L, Chen L, Guo X Q, Zhu H T, Lu Y G. The construction of a library of single segment substitution lines in rice (Oryza sativa L.) [J]. Rice Genetics Newsletter, 2004, 21: 85-87
[17]  Xi Z Y, He F H, Zeng R Z, Zhang Z M, Ding X H, Li W T, Zhang G Q. Development of a wide population of chromosome single segment substitution lines in the genetic background of an elite cultivar of rice (Oryza sativa L.)[J]. Genome, 2006, 49(5): 476-484
[18]  Li G X, Chen A H, Liu X, Wang W Y, Ding H F, Li J, Liu W, Li S S, Yao F Y. QTL detection and epistasis analysis for heading date using single segment substitution lines in rice (Oryza sativa L.) [J]. Journal of Integrative Agriculture, 2014, 13(11):2311-2321
[19]  McCouch S R, Teytelman L, Xu Y B, Lobos B K, Clare K, Walton M, Fu B, Maghirang R, Li Z, Xing Y, Zhang Q, Kono I, Yano M, Fjellstrom R, DeClerck G, Schneider D, Cartinhour S, Ware D, Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.) [J]. DNA Research, 2002, 9(6): 199-207
[20]  Matsumoto T, Wu J Z, Kanamori H, Katayose Y. The map-based sequence of the rice genome [J]. Nature, 2005, 436(7052): 793-800
[21]  黄朝锋, 张桂权. 水稻PSM标记的发展及抗虫基因的分子定位[J]. 分子植物育种, 2003, 1(4): 572-574
[22]  Zheng K L, Huang N, Bennett J, Khush G S. PCR based marker-assisted selection in rice breeding [M]. IRRI Discussion Paper Series No. 12. Manila, Philippines: International Rice Research Institute, 1995: 1-24
[23]  Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.) [J]. Molecular and General Genetics, 1996, 252(5): 597-607
[24]  李文涛, 曾瑞珍, 张泽民, 张桂权. 栽培稻F1花粉不育基因座S-b的PCR标记定位[J]. 植物学报,2002, 44(4): 463-467
[25]  Lee S, Kim J, Han J N, Han M J, An G. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS-LIKE 20 (SOC1/AGL20) ortholog in rice[J]. Plant Journal, 2004, 38(5): 754-764
[26]  Yano M, Kojima S, Takahashi Y, Lin H, Sasaki T. Genetic control of flowering time in rice, a short-day plant[J]. Plant Physiology, 2001, 127(4): 1425-1429
[27]  刘冠明, 李文涛, 曾瑞珍, 张桂权. 水稻亚种间单片段代换系的建立[J]. 中国水稻科学, 2003, 17(3): 201-204
[28]  王立秋, 赵永锋, 薛亚东, 张祖新, 郑用链, 陈景堂. 玉米衔接式单片段导入系群体的构建和评价[J]. 作物学报, 2007, 33(4): 663-668
[29]  蔡健, 廖秋平. 水稻单片段代换系对CMS-DA恢复力的鉴定和遗传分析[J]. 核农学报, 2013, 27(5): 576-583
[30]  Ryu C H, Lee S Y, Cho L H. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)-dependent flowering in rice [J]. Plant Cell and Environment, 2009, 32(10): 1412-1427
[31]  Bian X F, Liu X, Zhao Z G, Jiang L, Gao H, Zhang Y H, Zheng M, Chen L M, Liu S J, Zhai H Q. Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1[J]. Plant Cell Reports, 2011, 30(12): 2243-2254

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133