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青花菜BrERF2基因的RACE克隆与模拟酸雨胁迫下的表达分析

DOI: 10.11869/j.issn.100-8551.2015.11.2093, PP. 2093-2102

Keywords: 青花菜,ERF,RACE克隆,生物信息学,表达

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Abstract:

为探讨青花菜在模拟酸雨胁迫下ERF基因的表达变化,应用RACE克隆技术,克隆获得青花菜的ERF因的1条cDNA全长序列,命名为BrERF2。应用生物信息学方法,对该基因编码蛋白的氨基酸组成、理化性质、跨膜结构域、信号肽导肽、疏水性/亲水性、二级结构、亚细胞定位、保守结构域等方面进行了预测和分析。结果表明:获得cDNA全长为958bp,开放阅读框为804bp,编码267个氨基酸,Real-timePCR结果分析表明模拟酸雨胁迫下BrERF2基因的表达量发生变化,模拟酸雨胁迫初期表达量显著增大,随时间延长,又开始下降,表明其可能参与了青花菜抗酸雨胁迫的应答机制。本研究为青花菜的抗酸雨胁迫研究奠定了基础。

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