全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

高静水压对水稻种子萌发及同工酶的影响

DOI: 10.11858/gywlxb.2003.04.007, PP. 283-289

Keywords: 高静水压力,水稻种子,同工酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

利用不同静水压力和不同保压时间处理了籼稻粤香占种子,研究发现,随着压力的增加和保压时间的延长,水稻发芽率和成苗率明显下降,高压处理后的水稻在苗期的生长受到明显抑制,大约生长30d株高才赶上对照;聚丙烯酰胺凝胶电泳显示高静水压力抑制了种子幼苗的酯酶(Esterase)同工酶的表达,但对其它几种同工酶如过氧化物酶(Peroxidase)、超氧化物歧化酶(SuperoxiderDismutase)和苹果酸酶(MalicEnyme)等影响较小。利用同一压力和保压时间处理,不同类型的水稻(OryzasativaL.)品种种子,其中包括籼稻(粤香占和南京11)、粳稻(秋光和台中65)和中间类型Bulu稻(BuluGendjah和BuluRusa),结果发现所有类型水稻种子的发芽率和成苗率都显著降低,但因水稻类型的不同其影响程度也不同,籼型水稻南京11和粤香占的耐压性最弱,粳型水稻秋光和台中65最强,中间型Bulu水稻BuluGendjah和BuluRusa居中。上述结果表明,静水高压能够显著降低水稻的种子的发芽率和成苗率,抑制幼苗前期的生长和酯酶同工酶的表达。

References

[1]  Bradford M M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [J]. Anal Biochem, 1976, 72: 248-254.
[2]  Sun X L, Cai H W, Wang X K. A Method for Studing Electrophoretic Variation of Isozymess in Rice with Poly-Acrylamide Gel [J]. Chinese Journal Rice Science, 1996, 10(1): 43-50. (in Chinese)
[3]  孙新立, 才宏伟, 王象坤. 水稻同工酶聚丙烯酰胺凝胶电泳方法探索 [J]. 中国水稻科学, 1996, 10(1): 43-50.
[4]  Pan R C. The Rice Physiology [M]. Beijing: Science Press, 1979: 218-224. (in Chinese)
[5]  潘瑞炽. 水稻生理 [M]. 北京: 科学出版社, 1979: 218-224.
[6]  Jiao D M, Ji B H. Changes of the Photosynthetic Electron Transport and Pthotosynthetic Enzyme Activities of Two Rice Varieties under Photooxidation Condition [J]. Acta Agronomica Sinica, 1996, 22(1): 43-49. (in Chinese)
[7]  焦德茂, 季本华. 光氧化条件下两个水稻品种光合电子传递和光合酶活性的变化 [J]. 作物学报, 1996, 22(1): 43-49.
[8]  Pan R C, Ji B H. Plant Physiology (3rd ed) [M]. Beijing: High Education Press, 1998: 237-240. (in Chinese)
[9]  潘瑞炽, 董愚得. 植物生理学(第3版) [M]. 北京: 高等教育出版社, 1995: 237-240.
[10]  Shen T, Wang J Y. Plant Biochemistry (2nd ed)[M]. Beijing: High Education Press , 1990: 234-236. (in Chinese)
[11]  沈同, 王镜岩. 生物化学. 上册(第2版) [M]. 北京: 高等教育出版社, 1990: 234-236.
[12]  Panick G, Winter R. Pressure-Induced Unfolding/Refolding of Ribonuclease a-a Static and Kinetik Fourier-Transform Infrared Spectroscopy Study [J]. Biochemistry, 2000, 39: 1862-1869.
[13]  Desai G, Panick G, Zein M, et al. Pressure-Jump Studies of the Folding/Unfolding of trp Repressor [J]. J Mol Biol, 1999, 288: 461-475.
[14]  Kayo C, Smorawinaka M, Li L, et al. Comparison of the Gene Expression of Aspartate Bate-D-Smialdehyde Dehydrogenase at Elevated Hydrostatic Pressure in Deep-Sea [J]. Bacteria J Biochem, 1997, 121(4): 717-723.
[15]  Malone A S, Shellhammer T H , Courtney P D. Effects of High Pressure on the Viability, Morphology, Lysis, and Cell Wall Hydrolase Activity of Lactococcus Lactis Subsp Cremoris [J]. Appl Environ Microb, 2002, 68(9): 4357-4363.
[16]  Hu N S, Wan G X. The Technology and Application of Isoenzyme [M]. Changsha: Hunan Technology Press, 1985: 96-99. (in Chinese)
[17]  胡能书, 万贤国. 同工酶技术及其应用 [M]. 长沙: 湖南科学技术出版社, 1985: 96-99.
[18]  Siegal B Z, Galston A W. The Isoperoxidases of Pisum Sativum [J]. Plant Physiol, 1967, 42: 221-226.
[19]  Bai C K, Li G S, Peng C L, et al. Preliminary Study of Rice Mutants Induced by High Hydrostatic Pressure [J]. Journal of Tropical and Subtropical Botany, 2003, 11(2): 132-136. (in Chinese)
[20]  白成科, 李桂双, 彭长连, 等. 高静水压诱导水稻变异的初步研究 [J]. 热带亚热带植物学报, 2003, 11(2): 132-136.
[21]  Bai C K, Li G S, Peng C L, et al. High Hydrostatic Pressure Induced Changes of Chlorophyll Fluorescence Rice Mutant Lines [J]. Chinese Journal Rice Sciene, 2003, 17(3): 228-232. (in Chinese)
[22]  白成科, 李桂双, 彭长连, 等. 高静水压诱变水稻突变株系的叶绿素荧光特性变化 [J]. 中国水稻科学, 2003, 17(3): 228-232.
[23]  Li G S, Bai C K, Duan J. Effect of High Hydrostatic Pressure Treatment on Physiological Characteristics of Rice Plants ( Oryza sativa L. ) [J]. Chinese Journalof High Pressure Physics, 2003, 17(2): 122-128. (in Chinese)
[24]  李桂双, 白成科, 段俊, 等. 静水高压处理对水稻植株生理特性的影响 [J]. 高压物理学报, 2003, 17(2): 122-128.
[25]  Winter R. High Pressure Effects in Molecular Biophysics [A]. Proceedings of the International School of Physics "Enrico Fermi", Course CXLVII on High Pressure Phenomena [C]. Amsterdam: IOS, 2002: 413-453.
[26]  Panick G, Winter R. Pressure-Induced Unfolding/Refolding of Ribonuclease A-a Statics and Kinetik Fourier-Transform Infrared Spectroscopy Study [J]. Biochemistry, 2000, 39: 1862-1869.
[27]  Shantha D S, Doulas M C, Ana J, et al. High Resolution NMR Study of the Pressure-Induced Unfolding of Lysozyme [J]. Biochemistry, 1992, 31: 7773-7778.
[28]  Whelch T J, Farewell A, Neidhandt F C, et al. Stress Response of E Coli to Elevated Hydrostatic Pressure [J]. J Bacteriol, 1993, 175: 7170-7177.
[29]  Arroyo G, Sanz P D, Prestamo G. Effect of High Pressure on the Reduction of Microbial Populations in Vegetables [J]. J Appl Microbiol, 1997, 82(6): 735-742.
[30]  Xu S P, Liao Y P, Weng K N, et al. Pressure Induced Rice Mutation and Effects of High Hydrostatic Pressure on the Growth and Development of Rice [J]. Chinese Journal of High Pressure Physics, 2001, 15(4): 241-247. (in Chinese)
[31]  徐世平, 廖耀平, 翁克难, 等. 水稻压致变异和高压对水稻生长发育的影响 [J]. 高压物理学报, 2001, 15(4): 241-247.
[32]  Xu S P, Liao Y P, Xiao W S, et al. Effects of High Pressure on the Growth and Development of Rice [J]. Chinese Journal of High Pressure Physics, 1999, 13(Suppl): 58-63. (in Chinese)
[33]  徐世平, 廖耀平, 肖万生, 等, 高压对水稻生长发育的影响 [J]. 高压物理学报, 1999, 13(增刊): 58-63.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133