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高静水压处理水稻诱导稳定遗传变异系的DNA分析

DOI: 10.11858/gywlxb.2004.04.001, PP. 289-294

Keywords: 高静水压,水稻,变异,ISSR,RAPD

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Abstract:

用高压手段诱变水稻品种“毕粳38”,产生了两个稳定的可遗传的变异:变异1,植株分蘖减少,成熟期较原种提早20d;变异2,植株变高,分蘖增加,籽粒变长,类似于籼稻。对原种及两个变异进行了ISSR及RAPD分析,结果显示原种及两个变异在ISSR和RAPD的检测中多态性明显,分别为28.57%和45.98%。变异2的变异大于变异1;ISSR检测中原种与变异1的平均绝对距离系数为0.0303,与变异2的平均绝对距离系数为0.2613。RAPD检测中原种与变异1的平均绝对距离系数0.0845,与变异2的平均绝对距离系数为0.4167。研究结果表明:高静水压可以对高等植物的种子进行诱变,产生在表型上及DNA分子水平上均可稳定遗传的变异。研究结果首次从DNA分子水平上证明高静水压处理可以产生变异。继各种化学制剂、各种射线诱变等方法之后,高压不失为一种行之有效的新诱变方法。

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