全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

基于超临界流体技术制备葡萄糖脂质体的实验研究

DOI: 10.11858/gywlxb.2014.05.018, PP. 625-630

Keywords: 超临界流体,脂质体,葡萄糖,水溶性药物

Full-Text   Cite this paper   Add to My Lib

Abstract:

脂质体由于其特殊的结构和性能,是一种很有发展潜力的药物载体,在医药和化妆品等行业具有广阔的应用前景。超临界逆向蒸发法是用超临界流体代替有机溶剂制备脂质体的方法,具有对水溶性药物脂质体制备过程简单、包覆率高等特点。以葡萄糖为模型药物,利用该方法制备出了葡萄糖脂质体,并详细考察了不同工艺条件对脂质体粒径和包覆率的影响。结果表明:用超临界逆向蒸发法可成功地制备出最小粒径为290nm、包覆率最高可达41.3%的葡萄糖脂质体。压力、温度和平衡时间对粒径和包覆率都有较大的影响。压力在10~30MPa时,随压力的增加,脂质体粒径显著减小至某一值后,基本趋于稳定,而包覆率逐渐增加,达到25.7%~27.5%,随后包覆率逐渐减小|温度在35~65℃时,随温度的增加粒径显著减小至最小值,然后逐渐增大,而包覆率一直增大,最高可达41.3%|平衡时间在15~45min时,随平衡时间的增加,粒径相对稳定,而包覆率显著增加,超过45min之后,粒径显著增加,包覆率显著下降。

References

[1]  Nagayasu A, Uchiyama K. The size of liposomes: A factor which affects their targeting efficiency to tumors and therapeutic activity of liposomal antitumor drugs [J]. Adv Drug Delivery Rev, 1999, 40(1/2): 75-87.
[2]  Sun J K, Deng Y J, Cao J N. Research overviews of nanoliposomes [J]. Journal of Shenyang Pharmaceutical University, 2010, 27(12): 993-997, 1002. (in Chinese)
[3]  孙聚魁, 邓英杰, 曹金娜. 纳米脂质体研究进展 [J]. 沈阳药科大学学报, 2010, 27(12): 993-997, 1002.
[4]  Zuzanna D K, Agata D J. Liposomes as delivery systems for antibiotics [J]. Int J Pharmac, 2010, 387(1/2): 187-198.
[5]  Wang R L, Liu C M, Liu W. Research progress on preparation methods of liposome [J]. Academic Periodical of Farm Products Processing, 2007(8): 12-14. (in Chinese)
[6]  王瑞莲, 刘成梅, 刘伟. 脂质体制备方法的研究进展 [J]. 农产品加工?学刊, 2007(8): 12-14.
[7]  Deng L H, Wei M Y, Tang C Y, et al. Effects of lyophilization process and lyoprotectants on the quality of hydroxycamptothecine liposomes [J]. Chinese Journal of Pharmaceuticals, 2012, 43(1): 30-34. (in Chinese)
[8]  邓礼荷, 韦敏燕, 汤晨懿, 等. 冻干工艺及保护剂对羟基喜树碱脂质体质量的影响 [J]. 中国医药工业杂志, 2012, 43(1): 30-34.
[9]  Liu X Q, Wang J Y, Tong Y, et al. Liposome preparation technology and its research progress [J]. Chinese Pharmaceutical Journal, 2011, 46(14): 1084-1088. (in Chinese)
[10]  刘晓谦, 王锦玉, 仝燕, 等. 脂质体制备技术及其研究进展 [J]. 中国药学杂志, 2011, 46(14): 1084-1088.
[11]  Zhang Z Q, Zhu J B, Yao J, et al. Preparation of irinotecan liposomes by pH-gradient method [J]. Journal of China Pharmaceutical University, 2008, 39(4): 312-316. (in Chinese)
[12]  张自强, 朱家壁, 姚静, 等. pH梯度法制备伊立替康脂质体 [J]. 中国医科大学学报, 2008, 39(4): 312-316.
[13]  Li Z, Wang S N, Huang W W, et al. Preparation of irinotecan hydrochloride liposomes and quantification of its entrapment efficiency [J]. Journal of Shenyang Pharmaceutical University, 2011, 28(9): 687-690, 720. (in Chinese)
[14]  李哲, 王绍宁, 黄微葳, 等. 盐酸伊立替康脂质体的制备及包封率的测定 [J]. 沈阳药科大学学报, 2011, 28(9): 687-690, 720.
[15]  Cheng X D, Li X M, Gu L. Liposome research progress of water-soluble drug coating method [J]. West China Journal of Pharmaceutical Sciences, 2009, 24(2): 194-197. (in Chinese)
[16]  程晓丹, 李学明, 顾立. 脂质体对水溶性药物包封方法的研究进展 [J]. 华西药学杂志, 2009, 24(2): 194-197.
[17]  Xia F, Jin H Y, Zhao Y P, et al. Supercritical antisolvent-based technology for preparation of vitamin D3 proliposome and its characteristics [J]. Chinese Journal of Chemical Engineering, 2011, 19(6): 1039-1046.
[18]  Otake K, Imura T. Development of a new preparation method of Liposomes using supercritical carbon dioxide [J]. Langmuir, 2001, 17: 3898-3901.
[19]  Imura T, Otake K. Preparation and physicochemical properties of various soybean lecithin liposomes using supercritical reverse phase evaporation method [J]. Colloids and Surfaces B: Biointerfaces, 2003, 27: 133-140.
[20]  Otake K, Shimomura T. Preparation of liposomes using an improved supercritical reverse phase evaporation method [J]. Langmuir, 2006, 22: 2543-2550.
[21]  Chen S H, Zhan S P, Zhang J, et al. Experimental research and properties characterization of the preparation of glucose liposome with the supercritical reverse phase evaporation method [J]. Journal of Dalian University, 2012, 33(6): 37-40.(in Chinese)
[22]  陈淑花, 詹世平, 张晶, 等. 超临界逆向蒸发法制备葡萄糖脂质体实验及性能表征 [J]. 大连大学学报, 2012, 33(6): 37-40.
[23]  Dang Q F, Liu C S, Yan J Q, et al. The preparation and characterization of CMCS-coated VE liposome [J]. Journal of Functional Materials, 2011, 42(9): 1631-1634. (in Chinese)
[24]  党奇峰, 刘成圣, 颜景泉, 等. 羧甲基壳聚糖包覆VE脂质体的制备及理化性质研究 [J]. 功能材料, 2011, 42(9): 1631-1634.
[25]  Cevc C, Blume G. Lipid vesicles penetrate into intact skin owning to the transdermal osmotic gtadients and hydration force [J]. Biochim Biophys Acta, 1992, 1104(1): 226-232.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133