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静电纺丝制备壳聚糖/聚己内酯血管支架及表征

, PP. 104-108

Keywords: 壳聚糖,聚己内酯,血管支架,静电纺丝,内皮祖细胞

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Abstract:

结合壳聚糖(CS)和聚己内酯(PCL)二者的优点,以静电纺丝的方法制备了CS/PCL血管支架。采用SEM和电子万能试验机检测了该支架的结构和力学性能,将内皮祖细胞(EPCs)与该支架膜复合培养,评估了该血管支架维持细胞黏附、繁殖和分化的能力。SEM结果显示:通过静电纺丝可以得到多孔、类似于天然细胞外基质的直径约400nm的纤维微结构;当CS与PCL质量比为0.5时,静电纺丝所制备的CS/PCL血管支架弹性最大形变达到31.64%,应力-应变曲线显示其弹性变形能力较强;EPCs在CS/PCL血管支架黏附率可达95.1%,荧光显微镜观察结果也显示了CS/PCL血管支架利于细胞黏附、生长。

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