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草地学报  2014 

不同生态型的11个柳枝稷品种组织培养反应评价

DOI: 10.11733/j.issn.1007-0435.2014.03.021, PP. 579-585

Keywords: 柳枝稷,发芽率,愈伤诱导,愈伤组织形态,再生

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Abstract:

以美国从南到北11个不同生态型柳枝稷(PanicumvirgatumL.)品种的成熟种子为外植体,进行愈伤组织诱导和分化,对其愈伤组织各种形态及比例进行统计分析,以评价不同生态型柳枝稷的组织培养反应。结果表明低地型品种Alamo和Performer的种子发芽率和愈伤诱导率均较低,但组织培养中结构松脆、生长旺盛、色泽鲜亮、增殖较快、容易分化成苗的Ⅱ型愈伤组织比例高,容易再生成苗;虽然高地型品种Dacotach,Blackwell和Nebraska28等的愈伤诱导率和种子发芽率较高,但较难挑选出Ⅱ型愈伤组织,不易获得组培再生苗。本研究结果为柳枝稷的遗传改良和生物技术育种奠定基础。

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