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草地学报  2014 

自然环境下不同秋眠型苜蓿PHYA和PHYBmRNA表达量的变化

DOI: 10.11733/j.issn.1007-0435.2014.03.020, PP. 572-578

Keywords: 光周期,温度,光敏色素,转录调控,苜蓿

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Abstract:

为揭示光敏色素如何响应外界环境及作用机理,并探讨其与苜蓿(Medicago)秋眠性的关系,以田间自然生长条件下的不同秋眠类型紫花苜蓿标准对照品种Norseman(FD1),Archer(FD5)和CUF101(FD9)为材料,应用RT-PCR测定在6月、8月、9月、11月采集的叶片PHYA和PHYBmRNA含量。结果表明从6月到11月,随着光照时间的逐渐缩短和温度的降低,3个不同秋眠类型紫花苜蓿品种PHYA和PHYB的mRNA含量均呈上升趋势,且PHYAmRNA含量比PHYBmRNA的含量高;除了6月份,其他月份均是随着紫花苜蓿秋眠性的提高,PHYA和PHYBmRNA表达呈上调趋势,且不同秋眠类型间的差距越来越大。据此推测PHYA和PHYB在苜蓿响应外界环境改变过程中起一定的作用,二者基因的转录受光周期与温度因子的调控,且随着光周期的缩短与温度的降低其mRNA量升高,PHYA和PHYB很可能参与了光周期与温度对紫花苜蓿秋眠性的调控。

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