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草地学报  2014 

冷胁迫对2种垂穗披碱草生长和生理特性的影响

DOI: 10.11733/j.issn.1007-0435.2014.04.017, PP. 789-795

Keywords: 冷胁迫,膜脂过氧化,活性氧,抗氧化酶,垂穗披碱草

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Abstract:

低温是影响植物生长的外界限制因子之一。采用沙培法研究了冷胁迫对2种垂穗披碱草(Elymusnutans)(当雄垂穗披碱草(西藏野生)和甘南垂穗披碱草)生长与生理特性的影响。结果表明冷胁迫处理5d显著抑制2种垂穗披碱草的生长和叶绿素的合成,特别是甘南垂穗披碱草发生严重氧化损伤,电解质渗透率和丙二醛(MDA)含量分别比对照增加114.7%和124.6%,而当雄垂穗披碱草受伤害较轻,分别比对照增加18.2%和10.8%。冷胁迫也显著增加了甘南垂穗披碱草中H2O2和自由基的积累,而当雄垂穗披碱草与对照相比差异不显著。冷胁迫处理5d后,当雄垂穗披碱草的类胡萝卜素含量较甘南增加72.3%,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性分别较对照增加80.3%,27.3%和65.9%;甘南垂穗披碱草的SOD和POD活性分别较对照增加11.4%和28.9%,而其CAT活性下降25.5%。相关分析表明,垂穗披碱草在冷胁迫下细胞膜受损伤程度与抗氧化酶活性负相关。当雄垂穗披碱草通过提高抗氧化酶活性,可有效清除体内积累的活性氧,减轻细胞膜系统受损伤的程度从而提高其抗寒性。

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