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草地学报  2014 

高温胁迫对草地早熟禾抗氧化酶活性及其同工酶图谱的影响

DOI: 10.11733/j.issn.1007-0435.2014.06.025, PP. 1308-1317

Keywords: 草地早熟禾,高温胁迫,抗氧化酶,同工酶

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Abstract:

为阐明冷季型草坪草抗氧化酶对高温的胁迫响应,以草地早熟禾(Poapratensis)耐热品种‘热力’(ThermalBlue)、中耐热品种‘大师’(Utmost)和热敏感品种‘长征’(Excursion)为试验材料,动态研究45℃/40℃(昼/夜)高温胁迫下叶片中5种抗氧化酶活性的变化.结果表明45℃/40℃高温胁迫使得3个草地早熟禾品种的超氧化物歧化酶(SOD)活性升高;耐热品种‘热力’过氧化物酶(POD)活性呈先升高后降低的趋势,‘大师’和‘长征’POD活性变化不显著;3个草地早熟禾品种随高温胁迫时间的延长过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性明显降低;‘热力’抗坏血酸过氧化物酶(APX)活性在胁迫第1d和第1.5d出现显著降低,‘大师’APX活性胁迫第0.5d显著降低,而第1d显著增加,‘长征’胁迫1.5d出现显著升高,且3个草地早熟禾品种在胁迫第2d时APX活性均恢复到胁迫前的水平,与胁迫前相比差异不显著.同工酶表达上,3个草地早熟禾品种的SOD,POD和CAT同工酶表达量变化不显著,GR同工酶表达量减弱,APX同工酶表达量在耐热品种‘热力’中增加,在不耐热品种‘长征’中变化不大.

References

[1]  Wang Z, Huang B. Physiological recovery of Kentucky bluegrass from simultaneous drought and heat stress [J]. Crop Science,2004,44(5):1729-1736
[2]  Beard J B. Turfgrass: Science and culture [M]. New Jersey: Prentice-Hall Englewood Cliffs,1973
[3]  Chou M, Chen Y M, Lin C Y. Thermotolerance of isolated mitochondria associated with heat shock proteins[J].Plant Physiology,1989,89(2):617-621
[4]  王学奎. 植物生理生化实验原理和技术[M]. 第2版. 北京:高等教育出版社,2006:172-173
[5]  张志良. 植物生理学实验指导[M]. 北京:高等教育出版社, 2001:154-155
[6]  Aebi H. Catalase in vitro [J]. Methods Enzymol,1984,105:121-126
[7]  沈文飚,徐朗莱,叶茂炳,等. 抗坏血酸过氧化物酶活性测定条件的探讨[J]. 植物生理学通讯,1996,32(3):203-205
[8]  Halliwell B, Foyer C H. Properties and physiological function of a glutathione reductase purified from Spinach leaves by affinity chromatography [J]. Planta,1978,139(1):9-17
[9]  华子春,郑伟娟. 酶的凝胶电泳检测手册[M]. 北京:化学工业出版社,2008
[10]  汤章城. 现代植物生理学实验指南[M]. 北京:科学出版社,1999
[11]  Alscher R G, Heath L S. Role of Superoxide dismutases in controlling oxidative stress in plant [J]. Journal of Experimental Botany,2002,53(372):1331-1341
[12]  Abeliovich A, Kellenberg D, Shilo M. Effect of photooxidative conditions on levels of superoxide dismutase in Anacystis nidulans [J]. Photochemistry and photobiology,1974,19(5):379-382
[13]  Tanaka K, Sugahara K. Role of superoxide dismutase in defense against SO2 toxicity and an increase in superoxide dismutase activity with SO2 fumigation [J]. Plant and cell physiology,1980,21(4):601-611
[14]  Baum J A, Scandalios J G. Developmental expression and intracellular localization of superoxide dismutases in Maize [J].Differentiation,1979,13(2):133-140
[15]  Scandalios J G. Response of plant antioxidant defense genes to environmental stress [J]. Advances in genetics,1990,28:1-41
[16]  Almeselmani M, Deshmukh P S, Sairam R K, et al. Protective role of antioxidant enzymes under high temperature stress[J]. Plant Science,2006,171(3):382-388
[17]  Panchuk II, Volkov R A, Sch?ffl F. Heat stress-and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis[J].Plant Physiology,2002,129(2):838-853
[18]  Matters G L, Scandalios J G. Effect of the free radical-generating herbicide paraquat on the expression of the superoxide dismutase (SOD) genes in maize [J]. Biochimica et Biophysica Acta,1986,882(1):29-38
[19]  Scandalios J G. Oxygen stress and Superoxide Dismutases [J].Physiol Plant,1993,101(1):7-12
[20]  何亚丽,沈剑. 冷地型草坪草耐热机理初探:Ⅰ.草地早熟禾 (Poa pratensis.L)在热境胁迫下叶片叶绿素含量和POD酶活性的变化[J]. 上海农学院学报,1997,15(2):128-132
[21]  Wang Y, Tang X X, Li Y Q, et al. Antioxidant and isozyme features of two strains of Laminaria japonica(Phaeophyceae)[J].Chinese Journal of Oceanology and Limnology,2007,25(1): 67-72
[22]  张清,李琦明,崔西辰,等. 不同温度条件下两组辣椒的同工酶分析[J]. 上海大学学报:自然科学版,2004,10(5):488-492
[23]  He Y L, Huang B R. Differential Responses to heat stress in activities and isozymes of four antioxidant enzymes for two cultivars of Kentucky bluegrass contrasting in heat tolerance [J]. Journal of the American Society for Horticultural Science,2010,135(2):116-124
[24]  Gaspar T H, Penel C, Hagege D, et al. Peroxidases in plant growth, differentiation and development processes. In: Biochemical, Molecular and Physiological Aspects of Plant Peroxidases [D].Geneva:University of Geneve,1991:249-280
[25]  Jiang Y W, Huang B R. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation [J]. Crop Science,2001,41(2):436-442
[26]  栗淑媛,乔辰,扈瑞平,等. 螺旋藻POD,CAT,SOD同工酶的研究[J]. 微生物学通报,2004,31(1):82-85
[27]  李耀文. 水稻过氧化氢酶基因(CAT)同工酶在逆境胁迫下的比较分析[D]. 哈尔滨:东北林业大学,2009
[28]  鲁振强. 水稻APX与CAT同工酶的功能特性及其与盐胁迫关系的研究[D]. 哈尔滨:东北农业大学,2005
[29]  Anderson M D, Prasad T K, Stewart C R. Changes in isozyme profiles of catalase, peroxidase, and glutathione reductase during acclimation to chilling in mesocotyls of maize seedlings [J].Plant Physiology,1995,109(4):1247-1257
[30]  Asada K. Ascorbate peroxidase: A hydrogen peroxide scavenging enzyme in plants [J].Physiologia Plantarum,1992,85(2):235-241
[31]  Dash S, Mohanty N. Response of seedlings to heat-stress in cultivars of wheat: Growth temperature-dependent differential modulation of photosystem 1 and 2 activity, and foliar antioxidant defense capacity [J].Journal of plant physiology,2002,159(1):49-59
[32]  Foster J G, Hess J L. Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen [J]. Plant Physiology,1980,66(3):482-487

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