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草地学报  2014 

假俭草抗寒性状与低温胁迫响应EST分子标记的关联分析

DOI: 10.11733/j.issn.1007-0435.2014.06.024, PP. 1301-1307

Keywords: 假俭草,抗寒性状,EST分子标记,关联分析

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Abstract:

为深入研究抗寒分子机理以及为分子标记辅助育种奠定基础,以具有代表性的96份种源为材料,利用假俭草(Eremochloaophiuroides)抗寒品种TifBlair低温诱导后所得的EST及其近缘种高粱NCBI中低温胁迫响应的EST开发分子标记,对假俭草抗寒指标LT50观测值和EST分子标记位点进行关联分析.结果表明开发的17对引物在假俭草群体共扫描到46个多态遗传位点,其中CINAU114-900,CINAU115-1500和CINAU116-400位点与抗寒指标LT50有显著关联,其表型变异的解释率分别为5.10%,4.04%和4.59%;其LT50表型效应分别为0.2518℃,-0.3121℃和0.2449℃.CINAU114-900和CINAU116-400位点提高假俭草对低温的敏感性,而CINAU115-1500位点可提高其抗寒性.

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