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草地学报  2015 

丛枝菌根真菌对垂穗披碱草吸收不同氮源效率的影响

DOI: 10.11733/j.issn.1007-0435.2015.02.012, PP. 294-301

Keywords: 丛枝菌根真菌,垂穗披碱草,谷氨酸,有机氮

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Abstract:

为了研究丛枝菌根真菌(AM)对垂穗披碱草(Elymusnutans)吸收不同氮源效率的影响,通过在石英砂中不接菌、接种灭活真菌和激活真菌来探讨植株对谷氨酸和硝酸铵的吸收效率,以期为优质高产垂穗披碱草新品种选育及野生种质资源的开发利用提供理论指导.结果表明接种激活真菌处理的植株根系均受到不同程度的侵染,且侵染率随时间变化呈先增加后减少的趋势.在不同氮源条件下,接种AM真菌均能显著提高垂穗披碱草的叶绿素a、叶绿素b、总叶绿素、类胡萝卜素、总氮、总游离氨基酸、可溶性蛋白的含量和硝酸还原酶的活性(P<0.05),但对植株的叶绿素a与b的比值影响不大.当单独利用有机氮或无机氮作为氮源时,接种AM真菌能显著提高植株生物量,且在有机氮条件下效果更加明显.表明接种AM真菌有助于提高垂穗披碱草对有机氮吸收,增加牧草品质和产量.

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