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草地学报  2015 

蒙古冰草MwDREB3基因的克隆及表达分析

DOI: 10.11733/j.issn.1007-0435.2015.02.024, PP. 377-382

Keywords: 蒙古冰草,DREB,基因克隆,表达分析

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Abstract:

以蒙古冰草(Agropyronmongolicum)为研究材料,利用同源序列法克隆得到1个DREB3类转录因子基因,命名为MwDREB3,采用实时荧光定量RT-PCR技术分析MwDREB3基因的表达特征,为蒙古冰草优异抗性基因的挖掘和利用提供理论依据.结果表明该基因全长1056bp,包含1个完整的开放阅读框,长度为774bp,编码257个氨基酸,该蛋白近5'端含有1个保守的AP2结构域.系统进化树分析表明MwDREB3编码的蛋白与小麦(Triticumaestivum)的DREB、山羊草(Aegilopscolumnaris)DRF1的进化关系较近.该基因在干旱、高盐、ABA诱导条件下,表达量上调;在低温条件下,表达量下调.

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