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草地学报  2015 

青稞秸秆青贮饲料中优良乳酸菌的筛选及鉴定

DOI: 10.11733/j.issn.1007-0435.2015.03.025, PP. 607-615

Keywords: 青稞秸秆,青贮饲料,乳酸菌

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Abstract:

为筛选出适合于青藏高原青稞秸秆青贮用的优良乳酸菌菌株,研发新型青贮添加剂。分离了青藏高原青稞(Hordeumvulgare)秸秆青贮饲料中的乳酸菌,利用传统微生物培养法和16SrRNA序列分析法对乳酸菌进行鉴定,探讨其生物学特性,并筛选出优良的乳酸菌菌株。共分离获得4株同型发酵乳酸菌菌株,其中HG24,LH2为植物乳杆菌(Lactobacillusplantarum),LH9为乳酸片球菌(Pediococcusacidilactici),LH7为戊糖片球菌(Pediococcuspentosaceus)。分离出的乳酸菌菌株均能在5~30℃,pH3.5~7.0及3.0%和6.5%NaCl培养基中生长良好,其中HG24,LH2在pH值为3的培养基中仍能生长,体现出分离的乳酸菌菌株具有耐低温、耐酸性和耐盐性强的特征。4株乳酸菌菌株中HG24和LH2产酸能力强、生长速率快,最适宜于用作青贮乳酸菌菌种。

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