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草地学报  2015 

紫花苜蓿MsMYB2基因的克隆及转化

DOI: 10.11733/j.issn.1007-0435.2015.03.021, PP. 580-585

Keywords: 紫花苜蓿,MsMYB2基因,拟南芥,转化

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Abstract:

为获得MsMYB2基因过量表达的转基因拟南芥(Arabidopsisthaliana)植株,利用PCR技术在紫花苜蓿(Medicagosativa)中克隆出MYB2基因并命名为MsMYB2,MsMYB2基因编码区全长834bp,编码1条长度为278个氨基酸的多肽链。通过DNA重组技术将其与pBI121连接,成功构建了植物表达载体pBI-MsMYB2。通过花序侵染法获得具有卡那霉素抗性的转基因拟南芥植株,并通过PCR和RT-PCR对目的基因进行检测,结果证明目的基因已整合到拟南芥基因组中并且可以表达,成功获得了MsMYB2基因表达的拟南芥转基因植株。

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