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草地学报  2015 

蒙古冰草Afa家族串联重复序列克隆及染色体定位

DOI: 10.11733/j.issn.1007-0435.2015.03.002, PP. 448-452

Keywords: 蒙古冰草,Afa家族串联重复序列,克隆,荧光原位杂交

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Abstract:

Afa家族串联重复序列因只出现在小麦及小麦族近缘属物种而得名,本研究从蒙古冰草中克隆得到一个Afa家族序列,长度为233bp,命名为pAmAfa1,该序列在GENBANK中进行同源序列比对,结果表明该序列与大多数小麦族其他物种的Afa家族串联重复序列存在较高的相似性;系统进化分析表明,蒙古冰草pAmAfa1序列与大赖草pLrAfa3,pLrAfa5序列聚在一起,表明蒙古冰草P染色体组与大赖草的N、X染色体亲源关系较近。为了明确Afa家族串联重复序列在蒙古冰草染色体上的位置,双色荧光原位杂交技术被采用,以pAmAfa1为探针检测到杂交信号出现在染色体的末端或近端部的区域,每条染色体上都有杂交信号,表明Afa家族串联重复序列普遍存在于P染色体组中。

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