Hisano H, Nandakumar R, Wang Z Y. Genetic modification of lignin biosynthesis for improved biofuel production [J]. In Vitro & Developmental Biology-Plant,2009,45(3):306-313
[2]
Cao J, Yao D, Lin F, et al. PEG-mediated transient gene expression and silencing system in maize mesophyll protoplasts: A valuable tool for signal transduction study in maize [J]. Acta Physiologiae Plantarum,2014,36(5):1271-1281
[3]
Marion J, Bach L, Bellec Y, et al. Systematic analysis of protein subcellular localization and interaction using high-throughput transient transformation of Arabidopsis seedlings [J]. The Plant Journal,2008,56(1):169-179
Yang J W, Fu J X, Li J, et al. A novel co-immunoprecipitation protocol based on protoplast transient gene expression for studying protein-protein interactions in rice [J]. Plant Molecular Bology Reporter,2014,32(1):153-161
[6]
Sheen J. Signal transduction in maize and Arabidopsis mesophyll protoplasts [J]. Plant Physiology,2001,127(4):1466-1475
[7]
Davey M R, Anthony P, Power J B, et al. Plant protoplasts: Status and biotechnological perspectives [J]. Biotechnology Advances,2005,23(2):131-171
Zhang Y, Su J, Duan S, et al. A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes [J]. Plant Methods,2011,7(1):30
[11]
Cocking E C. A method for the isolation of plant protoplasts and vacuoles [J]. Nature Protocols,1960(187):962-963
[12]
Yoo S D, Cho Y H, Sheen J. Arabidopsis mesophyll protoplasts: A versatile cell system for transient gene expression analysis [J]. Nature Protocols,2007,2(7):1565-1572
[13]
Ueki S, Lacroix B, Krichevsky A, et al. Functional transient genetic transformation of Arabidopsis leaves by biolistic bombardment [J]. Nature Protocols,2009,4(1):71-77
[14]
Reich T J, Iyer V N, Miki B L. Efficient transformation of alfalfa protoplasts by the intranuclear microinjection of Ti plasmids [J]. Nature Biotechnology,1986,4(11):1001-1004
Vermeer J E M, Van Munster E B, Vischer N O, et al. Probing plasma membrane microdomains in cowpea protoplasts using lapidated GFP-fusion proteins and multimode FRET microscopy [J]. Journal of Microscopy,2004,214(2):190-200
[18]
Ali G S, Prasad K V S K, Hanumappa M, et al. Analyses of in vivo interaction and mobility of two spliceosomal proteins using FRAP and BiFC [J]. PLoS One,2008,3(4):e1953
Tang R, Zhang X Q, Li Y H, et al. Cloning and in silico analysis of a cinnamyl alcohol dehydrogenase gene in Pennisetum purpureum [J]. Journal of Genetics,2014,93(1):145-158
Bart R, Chern M, Park C J, et al. A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts [J]. Plant Methods,2006,2(1):13-21
