全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草地学报  2015 

不同浓度激素配比对高羊茅再生体系建立的影响

DOI: 10.11733/j.issn.1007-0435.2015.03.013, PP. 517-525

Keywords: 草坪草,组织培养,再生体系

Full-Text   Cite this paper   Add to My Lib

Abstract:

以‘爱瑞3号’为试材,以无菌种子为外植体,对成熟种子愈伤组织诱导、胚性愈伤组织继代和分化的影响因素进行了研究,构建了高频的高羊茅胚性愈伤组织植株再生体系。结果表明最佳愈伤诱导培养基配方为MS+5.5mg·L-12,4-D+500mg·L-1CH;最佳分化培养基配方为MS+2.0mg·L-16-BA+1.0mg·L-1KT;最佳生根培养基配方为1/2MS+0.3mg·L-1IBA,其蔗糖含量为20g·L-1。

References

[1]  Ashok C, Qu R. Somatic embryogenesis and plant regeneration of turf-type bermudag rass: Effect of 6-benzyl adenine in callus induction medium [J]. Plant Cell, Tissue and Organ Culture,2000,60(2):113-120
[2]  何阳鹏,于海涛. 不同种胚及激素处理对高羊茅愈伤组织诱导的影响[J]. 安徽农业科学,2003,31(3):371-374
[3]  高丽美,徐子勤,张永彦,等. 高羊茅组织培养再生体系及 GUS基因瞬间表达研究[ J] . 西北植物学报,2001,25(1):40-45
[4]  Zhao J, Zhi D, Xue Z, et al. Enhanced salt tolerance of transgenic Progeny of ta11 fescue(Festuca arundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis[J]. Journal of Plant Physiology,2007,164(10):1377-1383
[5]  胡章华,陈火庆,吴关庭, 等. 百慕大成熟胚的组织培养及植株再生[J] . 草业学报,2003,12(1):85-89
[6]  于晓,朱桢,付志明, 等. 提高小麦愈伤组织分化频率的因素[J] . 植物生理学报,1999,25(4):388-394
[7]  杨跃生,简玉瑜. 脱水处理对水稻组织培养植株再生的高效调控作用[J] . 农业科学集刊第二集(农作物原生质体培养专辑),1995:39-46
[8]  Barnes R F. Importance and problems of tall fescue. In: Kasperbauer MJ(ed).Biotechnology in tall fescue improvement [M\]. CRC Press,1990:2-12
[9]  Van der Valk P, Zeal M A. Creemers-Molenaar. Somatic embryogenesis and plant regeneration in inflorescence and seed derived callus cultures of Poa pratensis L. (Kentucky bluegrass)[J]. Plant Cell Report,1989,7(8):644-647
[10]  Van Ark H F, Zaa M A, Creemers-Molenaar J. et al. Improvement of the tissue culture response of seed-derived callus cultures of Poa pratensis L.:Effect of gelling agent and abscisic acid[J]. Plant Cell, tissue and Organ Culture,1991,27(3):275-280
[11]  马忠华,张云芳,徐传祥, 等.早熟禾的组织培养和基因枪介导的基因转化体系的初步建立[J]. 复旦学报(自然科学版),1999,10(5):540-544
[12]  丁路明, 龙瑞军, 王长庭. 肯塔基草地早熟禾愈伤组织的诱导及再生体系的建立[J]. 中国草地,2005,27(3):31-36
[13]  谭文澄,戴策刚. 观赏植物组织培养技术[M]. 北京:中国林业出版社,1991
[14]  Wang Z Y, Andrew H, Rouf M. Forage and turf grass biotechonology [J]. Critical Review in Plant Science,2001,20(6):573 - 619
[15]  Przetakiewice A, Orczyk W, Nadolaska-Orczyk A. The effect of auxin on plant regeneration of wheat, barley and triticale [J] . Plant Cell, T issue and Organ Culture,2003,73(3):245-256
[16]  Bai Y, Qu R. An evaluation on callus induction and plant regeneration of 25 turf-type tall fescue (Festuca arundinacea Schr eb.) cultivars[J] . Grass and Forage Science,2000,55(4):326-330
[17]  柴明良. 草坪草转基因研究进展[J]. 科技通报,2002,18(1):73-74
[18]  何勇, 田志红, 郑思琏. 高羊茅成熟胚离体培养及高频植株再生[J]. 草业科学,2005,22(6):23-29
[19]  Bai Y, Qu R. Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue[J]. Plant Breeding,2001,120(3):239-242

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133