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氧气与盐度对港西某油井本源微生物的影响

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Keywords: 本源微生物,氧气,盐度,PCRDGGE技术,微量量热技术

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Abstract:

为了探索绿色、环保、经济的微生物采油技术在大港港西某油井的基本理论参数,本文通过PCRDGGE技术和微量量热技术,开展了在好氧激活本源微生物技术下,氧气的进入对油藏本源微生物细菌群落的影响以及本源微生物生长的适宜盐度范围的试验研究。结果表明氧气的进入对油藏本源微生物细菌群落将构成很大的影响,在10d的培养期内,氧气表现出了很强的选择作用,其促进了一些好氧或兼性细菌的快速生长,同时也抑制了厌氧细菌的生长,此后不同时期的本源微生物细菌群落表现各不相同,但其多样性水平差异不大,可能是因为微生物代谢过程的连续反应、微生物之间的拮抗和协同作用等;微生物的热功率输出曲线因盐度的不同而表现出很强的规律性,通过对其相关参数的计算、拟合及分析,得到大港港西被测油井的本源微生物好氧激活生长的最佳盐度条件为2000~5000mg/L,较佳盐度条件为0~10000mg/L,而当盐度大于50000mg/L时,微生物活动基本停止,不适于好氧激活技术的应用。

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