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草业科学  2014 

建植年限对紫花苜蓿根际土壤微生物群落功能多样性的影响

DOI: 10.11829\j.issn.1001-0629.2013-0424, PP. 787-796

Keywords: 建植年限,紫花苜蓿,微生物群落,BiologMT

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Abstract:

?利用BiologMT技术,研究了3个紫花苜蓿(Medicagosativa)品种(肇东苜蓿、中苜一号和阿尔冈金)的建植年限(两年和10年)对根际土壤微生物碳代谢多样性的影响。结果表明,建植10年紫花苜蓿的根际土壤在表征土壤微生物代谢活性的平均颜色变化率(AWCD)上明显高于建植两年的根际土壤,其中10年阿尔冈金的AWCD值最高,而两年肇东苜蓿的值最低。相同品种不同建植年限间,建植10年紫花苜蓿的根际土壤微生物McIntosh均匀度指数明显高于建植两年,而10年和两年根际土壤微生物在Shannon多样性指数和SimpsonMT优势度指数上差异不显著。这说明建植10年的紫花苜蓿改变了根际土壤微生物群落的组成结构,同时也提高了根际土壤微生物群落的多样性。通过主成分分析,相同品种、不同建植年限下土壤微生物的碳源利用模式出现的差异并不显著,但10年紫花苜蓿根际土壤微生物间碳源利用的模式存在差异,主要碳源为糖类、羧酸和氨基酸等。

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