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草业科学  2014 

细胞分裂素调控的不同茬高黑麦草再生机制

DOI: 10.11829\j.issn.1001-0629.2013-0540, PP. 1310-1317

Keywords: 黑麦草,叶片再生,细胞分裂素,茬高

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Abstract:

?通过检测植物生长激素研究不同茬高多花黑麦草(Loliummultiflorum)叶再生的机制。试验结果表明,遮光条件下,高茬和高茬断根的再生叶片生物量均显著高于低茬的再生叶片生物量(P<0.05)。光照试验结果表明,低茬在单次去叶时、高茬在多次去叶时均易提高总生物量系数(总生物量系数表示单位茬质量所支撑的再生过程中总有机物质量的变化,是用来评价非贮存有机物质参与叶片的再生能力,是不同茬高黑麦草再生能力强弱的指标)。通过检测光照条件下的叶片和伤流液中玉米素+玉米素核苷(Z+ZR)及脱落酸(ABA)含量,发现叶Z+ZR含量与总生物量系数相关密切,且叶Z+ZR含量受根系的直接调控。基于根系对叶片的影响,根系诱导的叶片细胞分裂素是影响不同茬高黑麦草再生的关键因素。

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