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草业科学  2014 

基于ITS2序列的17种外来入侵植物分子鉴定

DOI: 10.11829\j.issn.1001-0629.2013-0697, PP. 1900-1907

Keywords: ITS2,外来植物,生物入侵,分子鉴定

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Abstract:

?中国入侵植物种类繁多、危害严重,对入侵物种的准确鉴定是开展各项研究的基础。本研究采用植物DNA条形码序列ITS2片段来探讨其在入侵植物中的鉴定能力。结果表明,ITS2序列在入侵物种及其近缘物种间存在明显的遗传差异,种间变异大于种内变异,在17种常见入侵植物与36种近缘物种的300份样品鉴定中成功率达95.5%。构建的NJ树可以很好地显示各不同入侵植物物种的聚类。此外,ITS2的二级结构也具有一定的系统学及分类学意义。因此,ITS2条形码序列能够作为入侵植物分子鉴定的有力工具,具有重要的应用价值。

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