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草业科学  2015 

Thidiazuron对解除羊草种子休眠和植株再生的影响

DOI: 10.11829\j.issn.1001-0629.2015-0189, PP. 1612-1618

Keywords: 羊草,TDZ,休眠,再生,超微观察

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Abstract:

?研究了不同浓度植物生长调节剂噻苯隆(Thidiazuron,TDZ)对解除羊草(Leymuschinensis)种子休眠和植株再生的影响,并对愈伤组织进行超微结构观察。结果表明,0.02mg·L-1TDZ低温浸种对解除羊草种子休眠和诱导胚性愈伤组织的效果最佳,发芽率和出愈率分别为11.0%和15.5%;羊草愈伤组织分化最佳培养基配方为MS+TDZ0.5mg·L-1+CH2.0g·L-1,分化率、绿芽分化率分别为56.7%和423.3%;TDZ低温浸泡处理的羊草种子诱导出的胚性愈伤组织较非胚性愈伤组织细胞质体中内含物丰富,更易于体胚形成;丛生芽经过1/2MS生根培养基的3次继代,炼苗移栽后成活率达100%。

References

[1]  Chupeau M C,Lemoine M,Chupeau Y.Requirement of TDZ for healthy protoplast development to efficient tree regeneration of a hybrid poplar (Populus tremula×P. alba)[J].Journal of Plant Physiology,1993,141:601-609.
[2]  李卫,董文,赵东利,赵东利,郭光沁,郑国锠.TDZ诱导甘露子茎段高频再生[J].西北植物学报,2002,22(4):965-969.
[3]  Emst R.Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae)[J].Plant Cell,Tissue and Organ Culture,1994,39(3):273-275.
[4]  陈肖英,叶庆生,刘伟.TDZ研究进展(综述)[J].亚热带植物科学,2003,32(3):59-63.
[5]  王亚馥,崔凯荣,汪丽虹,王伦山.小麦体细胞胚发生的超微结构研究[J].植物学报,1994,36(6):418-422.
[6]  黄泽豪,朱锦懋,母锡金,林金星.羊草有性繁殖力低的成因研究进展[J].中国草地学报,2002,24(6):55-60.
[7]  蔺吉祥,邵帅,隋丹,穆春生,王永翠,王颖.几种提高羊草种子发芽率方法的比较[J].中国草地学报,2014,36(3):47-51.
[8]  黄晓辉,胡小文,徐宗海,王彦荣.羊草种子休眠和萌发的激素调控研究[J].草业学报,2013,22(5):183-189.
[9]  Murthy B N S,Murch S J,Saxena P K.Thidiazuron:A potent regulator of in vitro plant morphogenesis[J].In Vitro Cellular & Developmental Biology-Plant,1998,34(4):267-275.
[10]  Saxena P K,Malik K A,Gill R.Inducition by TDZ of somatic embryogenesis in intact seedlings of peanut[J].Plant Physiology,1992,187:421-424.
[11]  Visser C,Quresni J A,Gill R.Morphoregulatory role of TDZ Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyls cultures[J].Plant Physiology,1992,99:1704-1707.
[12]  Chhabra G,Chaudgary D,Varma M.TDZ-induced direct shoot organogenesis and somatic embryogenesis on cotyledonary node explants of lentil (Lens culinaris Medik.)[J].Physiology and Molecular Biology of Plants,2008,14(4):347-353.
[13]  Luis P B C,Adriane C M G M, Sikvia B R C C,Ana C M B.Plant regeneration from seedling explants of Eucalyptus grandis×E.urophylla[J].Plant Cell,Tissue and Organ Culture,1999,56(1):17-23.
[14]  Gallo-Meagher M,English R G,Abouzid A.Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus[J].In Vitro Cellular & Developmental Biology-Plant,2000,36(1):37-40.
[15]  Shan X Y,Li D S,Qu R D.Thidiazuron promotes in vitro regeneration of wheat and barley[J].In Vitro Cellular & Developmental Biology-Plant,2000,36(3):207-210.
[16]  徐晓峰,黄学林.TDZ:一种有效的植物生长调节剂[J].植物学通报,2003,20(2):227-237.
[17]  张月琴,陈耀锋,王晶晶,王娜,王丽.TDZ预处理及激素诱导对小麦茎尖丛生芽形成的影响[J].麦类作物学报,2014,34(7):990-996.
[18]  郎文香,李宏博,孙丹,傅俊范,朴钟云.离体条件下TDZ对解除北五味子种子休眠的影响[J].西北植物学报,2010,30(9):1871-1875.
[19]  张彦琴,梁改梅,杨丽莉,刘晶玉,郭先龙,常建忠,曲俊民.橄榄球2号草地早熟禾愈伤组织的诱导和植株再生[J].山西农业科学,2015,43(1):7-9.
[20]  赵永英,赵献林,相志国,康明辉,张丹.TDZ在小麦花药培养中的作用[J].麦类作物学报2015,35(2):159-166.

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