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草业科学  2015 

象草PpCCR基因正、反义表达载体的构建及对烟草的转化

DOI: 10.11829\j.issn.1001-0629.2014-0572, PP. 1586-1593

Keywords: 象草,CCR基因,表达载体,遗传转化,烟草

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Abstract:

?象草(Pennisetumpurpureum)是一种广泛种植于热带和亚热带地区的多年生资源植物,但其木质素含量过高,影响了对其的开发与利用。为探讨肉桂酰辅酶A还原酶(Cinnamoyl-CoAReductase,CCR)基因对木质素合成的影响,本研究以华南象草为材料,构建了CCR基因正反义表达载体,并利用农杆菌侵染法转化烟草。通过Wiesner和Maule染色法对转基因植株进行木质素的组织化学染色。结果发现,转基因植株的木质素单体组成并没有明显改变;但PpCCR过表达烟草,转基因株系木质化细胞增多;PpCCR反义表达烟草,转基因株系木质化细胞排列相对松散。同时反义表达CCR,在木质素含量下降最明显的植株中茎秆呈红褐色。上述结果表明,CCR对木质素的合成起重要作用,这为进一步对象草进行遗传改良奠定了基础。

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