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草业科学  2013 

绒山羊细管冻精的解冻方法

, PP. 2066-2070

Keywords: 解冻方法,细管冻精,精子品质,,绒山羊

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Abstract:

?将6只2周岁的辽宁绒山羊种公羊精液混合后制成0.25mL细管冻精,分别采用37℃12s、39℃8s、70℃4s3种方法进行解冻,通过对精子活力、精子存活时间、顶体完整率、畸形率和精清酶进行分析,并结合升温曲线,研究解冻方法对绒山羊细管冻精品质的影响。结果发现,70℃解冻4s的精子活力显著高于37℃12s(P<0.05),与39℃8s相比差异虽不显著(P>0.05),但亦有增高的趋势;不同解冻方法对解冻后精子存活时间的影响差异不显著(P>0.05);70℃解冻4s的精子顶体完整率显著高于其余两种解冻方法(P<0.05),且精子畸形率显著低于其余两种解冻方法(P<0.05);70℃解冻4s的精清中GOT活性显著低于其余两种解冻方法(P<0.05),且LDH活性和HYD含量均显著低于37℃12s(P<0.05);70℃下解冻4s的精子经过危险温区速率最快。因此,70℃4s解冻方法能有效缓解解冻对精子的损伤,对保持精子的正常形态和活力较为有利,是绒山羊细管冻精适宜的解冻方法。

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