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OALib Journal期刊
ISSN: 2333-9721
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嗜碱Bacillussp.N16-5不同碳源条件下比较蛋白质组分析
, PP. 1117-1127
Keywords: 嗜碱Bacillussp.N16-5,嗜碱微生物,胞浆蛋白质,2-DE
Abstract:
为了解碳水化合物对嗜碱微生物代谢途径的影响,用蛋白质组学方法比较分析了不同碳源条件下培养的嗜碱菌的胞浆蛋白质变化,试图找到差异表达的蛋白.分离自内蒙古乌杜淖尔碱湖的嗜碱Bacillussp.N16-5,在含有5种不同碳源(葡萄糖、甘露糖、半乳糖、阿拉伯糖和木糖)的培养基中培养.比较蛋白质组学分析鉴定了61个差异表达蛋白,它们主要参与碳水化合物代谢、氨基酸转运和代谢、能量的产生和贮存.结果表明,不同碳水化合物条件下参与中央代谢途径酶的丰度发生了很大的变化,尤其是碳代谢调控蛋白A(CcpA)均被上调.同时发现,在CcpA参与调控的碳代谢抑制现象中戊糖表现出比己糖更强的效应.上述结果为进一步理解嗜碱微生物碳水化合物代谢奠定了基础.
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