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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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硅化超顺磁氧化铁纳米颗粒标记人羊膜间充质细胞的效率及其对细胞增殖的影响
, PP. 1154-1160
张可华, 郭丽丽, 蔡哲, 潘琳, 程立明, 舒峻, 李荣旗, 陈瀛, 欧华, 黄小杰, 张向利, 张红霞, 刘佳新, 左和鸣, 徐扬, 高艳, 崔晓惠
Keywords: 人羊膜间充质细胞,超顺磁性氧化铁纳米颗粒,细胞标记
Abstract:
旨在探讨一种新型硅化超顺磁性氧化铁纳米颗粒标记人羊膜间充质细胞的最佳方法,并检测其对细胞增殖的影响.用不同浓度的Si-SPIO和多聚赖氨酸混合制备PLL-Si-SPIO复合物,标记体外培养的hAMCs.利用普鲁士蓝染色和透射电子显微镜等方法对Si-SPIO的标记情况进行分析鉴定.分析Si-SPIO标记后1~4周铁颗粒在细胞内的维持与稳定.应用MTS分析法探讨经Si-SPIO标记后hAMCs的增殖活性.Si-SPIO标记后的hAMCs移植到小鼠纹状体内1周,鉴定Si-SPIO阳性细胞的存活与分布.观察发现,hAMCs经Si-SPIO标记后细胞内可检测到大量铁颗粒,铁颗粒能在细胞内维持4周以上.Si-SPIO标记具有浓度依赖性,最适浓度为20μg/mL;较低浓度的Si-SPIO对细胞增殖活力没有显著影响.移植到小鼠脑内1周后可见Si-SPIO阳性细胞.结果可知,浓度为20μg/mL的Si-SPIO标记hAMCs可获得良好的标记效果,并且不影响细胞的增殖活力.
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