OALib Journal期刊
位点特异性重组系统的机理和应用
, PP. 1090-1111
Keywords: 位点特异性重组 ,酪氨酸重组酶 ,丝氨酸重组酶 ,整合酶
Abstract:
位点特异性重组酶识别特定的位点形成联会复合体,并发生DNA链的切割与交换,实现靶位点之间的整合、切离或倒位.这一过程由位于重组酶催化活性中心的酪氨酸或丝氨酸向DNA磷酸骨架发起攻击,形成共价中间体,不需要高能量辅助因子的参与.由于位点特异性重组系统具有高效精确的优点,在基因工程领域得到了广泛的应用.本文从识别位点的性质、重组酶的组成与结构及催化反应的特点三方面对位点特异性重组的机理进行了全面的阐述,并对当前重组酶的应用研究之现状、热点及存在的问题作了深入剖析,并讨论了未来的发展趋势.
References
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