OALib Journal期刊
构建大肠杆菌药敏试验新方法的探索
, PP. 1063-1073
Keywords: 药敏试验 ,大肠杆菌 ,抗菌药物 ,递推系数
Abstract:
基于对大肠杆菌CVCC249菌株生长动力学过程的分析表明,导致药敏试验结果不确定性的原因主要是由于不能测计“药物浓度与菌群数量的比值”及“药物浓度及其作用时间的复合作用”对菌群生长动力学过程的影响所致.依据菌群在一系列不同浓度的庆大霉素及其作用时间下生长受到抑制的动力学过程分析和基于差分方法,即用简单的差分计算代替复杂的微分方程的数值解法的应用,提出了一个药敏试验的新方法:用菌群浊度值的净增量以消除休止细胞存在的影响后,以菌群浊度数列递推系数值的变化,表征药物对菌群增殖的影响,并结合用等值线图直观显示和分析药物浓度及其作用时间复合作用的方法.抗菌药物的抑菌率可表征为药物浓度及其作用时间的复合函数的动态变化,表明一类抗菌药物对某一菌株抑菌率的时间或浓度依赖性,取决于其浓度和存在时间的复合效应.用属于不同抑菌作用机理类型的恩诺沙星、左氧氟沙星和头孢曲松钠进行对比测试,检验了这一新方法的有效性.
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