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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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铜绿假单胞菌耐药性相关基因的筛选及鉴定
, PP. 978-982
Keywords: 铜绿假单胞菌,转座突变,耐药性,基因组
Abstract:
铜绿假单胞菌在人体内能引起严重的感染.由于铜绿假单胞菌高水平的内在性和获得性耐药性,使其引起的感染难以治愈.为解决该问题,弄清病原菌抗生素抗性的分子机制是一个关键.本实验构建了含有17000个铜绿假单胞菌转座突变株文库,并在LB固体平板上,用7种抗生素在最小抑制浓度(MIC)和1/2MIC条件下,筛选抗生素抗性发生变化的突变株.结果确定了43株转座突变株,每个突变株对至少一种抗生素的敏感性表现出增加3倍或降低1/2的表型.通过随机PCR和DNA测序,确定了这些铜绿假单胞菌突变体中转座子在基因组上的插入位点,确定了被破坏的基因.其中,9个是已知的与抗生素抗性相关基因,包括mexI,mexB和mexR;24个是以前未知与抗性相关的基因,包括一个菌毛合成基因pilY1,这个菌毛合成基因的破坏导致菌株对羧苄青霉素的MIC增加了128倍.此外,43个基因中有12个是功能完全未知的基因.这些基因的筛选鉴定有助于了解铜绿假单胞菌中抗生素抗性的产生机制,这些基因或许可成为控制或扭转抗生素抗性的作用靶点.
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