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拟南芥AtMPK3启动子应答逆境信号的表达模式和必需区域分析

, PP. 963-969

Keywords: 拟南芥,AtMPK3,启动子,逆境

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Abstract:

蛋白激酶AtMPK3参与MAPK级联途径,在植物逆境信号转导中起重要作用.为深入研究AtMPK3基因在转录水平上应答各种环境胁迫的分子机制,本研究从拟南芥基因组中分离了AtMPK3基因转录起始位点上游1016bp的启动子序列,对其进行了生物信息学分析.对该启动子进行了系列缺失突变,并将完整启动子和缺失启动子片段与GUS报告基因融合,转基因导入到拟南芥中.携带AtMPK3启动子及其各种缺失突变体的转基因植株在干旱、高盐、低温、机械损伤等胁迫条件的GUS组织化学染色和荧光定量分析表明,AtMPK3启动子应答干旱、高盐、低温、机械损伤逆境信号的必需元件位于启动子序列中转录起始位点上游?188~?62区域内,揭示了AtMPK3启动子在不同环境胁迫条件下的表达模式的差异.本研究结果有助于阐述AtMPK3基因在转录水平上应答不同胁迫信号分子机制.

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