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OALib Journal期刊
ISSN: 2333-9721
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隐睾不育的分子基础
, PP. 899-908
Keywords: 隐睾,精子发生,细胞凋亡,热激,支持细胞,血睾屏障,紧密连接
Abstract:
隐睾症或热局部处理猴和大鼠睾丸,能引起可逆性生精细胞凋亡,出现少精或无精现象.43℃局部热浴猴睾丸可引起精液中精子数量发生可逆性减少.睾丸支持细胞为生精细胞提供结构支持与营养供给.生精上皮中支持细胞间以及支持细胞和各级生精细胞间的特殊连接在精子发生中起着至关重要的作用.本研究组发现,热处理后紧密连接分子,如occludin,zonulaoccludens-1(ZO-1)在24~48h表达明显下降,血睾屏障(blood-testisbarrier,BTB)发生了可逆性破坏.该过程还伴随着TGF-β2和TGF-β3表达增高,p38MAPK和ERK/MAPK信号通路激活.由此推测,热激可能通过引发TGF-bs增高,下调紧密连接相关蛋白的表达,导致细胞连接减弱,从而引起BTB结构发生可逆性紊乱.此外,本文还综述了成年小鼠实验性隐睾睾丸中总基因的表达变化,成功克隆了几个生理功能显著,与精子发生特异相关的新基因.
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