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禁饲和重新饲喂对鸡骨骼肌中MAFbx和MuRF1基因表达水平的影响

, PP. 1162-1166

Keywords: 禁饲,基因表达,MAFbx,MuRF1,MyoD,M-CK

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Abstract:

对禁饲和重新饲喂对鸡骨骼肌中蛋白酶体相关基因及其下游靶基因表达水平的影响进行了研究.MAFbx和MuRF1基因作为泛素-蛋白酶体系统的E3连接酶发挥生物学作用.对七日龄鸡分别禁饲24,48和48h后重新饲喂4h.测定了上述两个基因mRNA和蛋白质水平的表达.MAFbx和MuRF1的表达量随着禁饲时间的延长而上升,并且这种上升趋势随着重新饲喂而下调.同时测定了上述两种E3连接酶的靶蛋白,即MyoD和M-CK的表达量,这两种蛋白的表达量随禁食的延长而下降,并且这种下降趋势会被重新饲喂所阻止.这些结果表明,禁饲促进了MAFbx和MuRF1的表达,并可能导致相应靶蛋白的减少.

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