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HIV-1病毒样颗粒疫苗的构建及免疫原性研究

, PP. 1148-1154

Keywords: HIV-1,共转染,稳定表达细胞系,病毒样颗粒(VLPs),疫苗

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Abstract:

人免疫缺陷病毒HIV在全球的迅猛传播使得有效疫苗的研制工作变成重中之重.出于安全考虑,减毒或灭活的HIV病毒不能作为疫苗应用,VLPs则因为不具有病毒基因组而带来的安全性成为一个极具吸引力的选择.本研究通过共转染筛选得到了一个有效而持久表达HIV-1结构蛋白gag和env的真核细胞系,证实了细胞培养上清中的gag和env能够自组装成为病毒颗粒.这些VLPs能够在不经佐剂加强的条件下诱导产生具有特异性的体液和细胞免疫应答.

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