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γ射线对小鼠调节性T细胞功能及相关细胞因子的影响及其意义

DOI: 10.1360/052011-570, PP. 951-957

Keywords: 电离辐射,调节性T细胞,IL-2,IL-10,TGF-β

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Abstract:

调节性T细胞(regulatoryTcells,Tregs)及相关细胞因子在机体免疫平衡的调节中发挥重要作用,而其在放射免疫损伤中的作用尚不明确.本实验以6Gyγ射线照射C57BL/6小鼠,于照射后1~28d不同时间,检测外周血、胸腺和脾脏Treg细胞亚群及血清中细胞因子IL-2,IL-10及TGF-β含量的变化,以探讨其在放射免疫损伤中的作用机制.结果显示,小鼠经6Gyγ射线照射后各组织CD4+CD25+Treg细胞比例明显增加(P<0.05或P<0.01),胸腺CD4+CD25+Foxp3+Treg细胞比例于照后1d即明显增高(P<0.01),而在照后7d明显低于未照射组(P<0.01);血清抑制性细胞因子IL-10(7d),TGF-β(3d)含量明显增高(P<0.05),而IL-2浓度持续降低.本文揭示了Treg细胞及其相关细胞因子与辐射所致免疫功能受抑和免疫调节功能失衡密切相关,为进一步的辐射损伤机制研究奠定基础.

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