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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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应用BIGIS-4测序系统完成Glaciecolamesophilasp.nov.的全基因组测序和组装
, PP. 544-549
袁丽娜, 任鲁风, 李运涛, 韩伟静, 俞勇, 楚亚男, 刘贵明, 于丹, 滕明静, 王亮, 王绪敏, 周晓光, 俞育德, 于军
Keywords: Glacielolamesophilasp.nov.,BIGIS-4测序系统,海洋环境的适应
Abstract:
BIGIS-4是中国新一代基于焦磷酸测序技术的测序仪.本实验利用BIGIS-4完成了对Glacielolamesophilasp.nov.(Gmn)的全基因组测序.Gmn是一株从海洋无脊椎动物体内分离到的革兰氏阴性菌.BIGIS-4测序得到152043个高质量的测序片段,平均读长406bp.测序片段由BIGIS-4系统后处理模块组装.除单核苷酸同聚体引起的测序错误外,测序结果中没有检测到其他低质量错误.Gmn基因组全长5144318bp,共注释得到4303个基因,其中有大量的代谢基因,与菌种在海洋表面非脊椎动物的生长环境相关.Gmn的冷适应和信号转导相关基因为其对海洋低温环境的适应提供了依据.
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