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人参皂苷IH901抗肝癌生长及其侵袭转移的作用机理

, PP. 219-225

Keywords: 人参皂苷IH901,肝癌,凋亡,侵袭转移,作用机理

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Abstract:

人参皂苷IH901是一种天然二醇组人参皂苷肠道细菌最终代谢产物.目前研究表明,IH901具有抗肝癌活性,然而其作用机理尚不清楚.采用人肝癌细胞株SMMC-7721,HepG2,MHCC97-H来建立体内外的抗肿瘤模型,以探讨人参皂苷IH901的抗肝癌活性及其作用机理.结果发现,人参皂苷IH901能够通过细胞周期中亚二倍体峰的增加、线粒体跨膜电位(ΔΨm)崩溃、DNA梯形条带的形成和提高Caspase-9/Caspase-3的表达量、促进凋亡来抑制肝癌细胞的生长.通过抑制细胞的迁移和降低VEGF/bFGF的表达来抑制肝癌细胞的侵袭转移.裸鼠体内实验均表明了人参皂苷在体内外的抗肝癌活性,并且在分子、细胞、动物水平初步揭示了IH901抗肝癌生长及其侵袭转移的作用机理,为其作为抗肝癌药物的开发提供理论支持.

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