全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...

乙肝纤维化分级相关血浆蛋白标志物的定量蛋白质组筛选

, PP. 178-186

Keywords: 定量蛋白质组学,肝脏纤维化,标志物,血浆,乙型肝炎

Full-Text   Cite this paper   Add to My Lib

Abstract:

非创伤性肝纤维化检测对于评价肝炎进程、临床治疗以及药效评价等方面,都具有非常重要的作用.通过N末端标签的蛋白质组定量手段,从血浆中筛选肝纤维化分级相关的蛋白候选标志物.首先利用目前纤维化诊断黄金标准——肝穿病理检测,获得纤维化不同分级的合格样本血浆,并对肝纤维化各级间的血浆混合样本进行了蛋白质组定量比较分析,发现了72个与乙肝纤维化分级呈明显相关性的血浆蛋白,构成了一个有重要参考价值的、纤维化分级诊断的候选蛋白标志物库.用WB法进一步验证了定量结果的准确性.利用IPA在线分析后获得了4个生理代谢网络图,提示这些差异蛋白在肝纤维化进程中代表的不同功能影响.总之,利用N末端标签定量蛋白质组技术,全面定量挖掘了血浆中与乙肝纤维化分级相关的差异蛋白及变化规律,对于加深肝纤维化发病机制的认识和理解具有很大帮助,同时也为肝脏纤维化临床诊断和治疗提供有益信息和参照.

References

[1]  4 Friedman S L. Liver fibrosis-- from bench to bedside. J Hepatol, 2003, 38: S38-53
[2]  5 Smith J O, Sterling R K. Systematic review: non-invasive methods of fibrosis analysis in chronic hepatitis C. Aliment Pharmacol Ther, 2009
[3]  6 Beretta L. Comparative analysis of the liver and plasma proteomes as a novel and powerful strategy for hepatocellular carcinoma biomarkerdiscovery. Cancer Lett, 2009
[4]  7 Ong S E, Mann M. Mass spectrometry-based proteomics turns quantitative. Nat Chem Biol, 2005, 1: 252-262??
[5]  8 Tao W A, Aebersold R. Advances in quantitative proteomics via stable isotope tagging and mass spectrometry. Curr Opin Biotechnol, 2003,14: 110-118??
[6]  9 Wu S, Kaiser N K, Meng D, et al. Increased protein identification capabilities through novel tandem MS calibration strategies. J ProteomeRes, 2005, 4: 1434-1441
[7]  10 Scheuer P J. Classification of chronic viral hepatitis: a need for reassessment. J Hepatol, 1991, 13: 372-374??
[8]  11 Beardsley R L, Reilly J P. Optimization of guanidination procedures for MALDI mass mapping. Anal Chem, 2002, 74: 1884-1890??
[9]  12 Ji C, Guo N, Li L. Differential dimethyl labeling of N-termini of peptides after guanidination for proteome analysis. J Proteome Res, 2005,4: 2099-2108??
[10]  13 Jia N, Liu X, Wen J, et al. A proteomic method for analysis of CYP450s protein expression changes in carbon tetrachloride induced male ratliver microsomes. Toxicology, 2007, 237: 1-11??
[11]  14 Meneses-Lorente G, Watt A, Salim K, et al. Identification of early proteomic markers for hepatic steatosis. Chem Res Toxicol, 2006, 19: 986-998??
[12]  2 Lok A S, McMahon B J. Chronic hepatitis B. Hepatology, 2001, 34: 1225-1241??
[13]  15 Nedelkov D, Kiernan U A, Niederkofler E E, et al. Investigating diversity in human plasma proteins. Proc Natl Acad Sci USA, 2005, 102:10852-10857??
[14]  3 Afdhal N H, Nunes D. Evaluation of liver fibrosis: a concise review. Am J Gastroenterol, 2004, 99: 1160-1174??
[15]  16 Lackner C, Struber G, Liegl B, et al. Comparison and validation of simple noninvasive tests for prediction of fibrosis in chronic hepatitis C.Hepatology, 2005, 41: 1376-1382??
[16]  17 Rockey D C, Bissell D M. Noninvasive measures of liver fibrosis. Hepatology, 2006, 43: S113-S120??
[17]  18 Everley P A, Bakalarski C E, Elias J E, et al. Enhanced analysis of metastatic prostate cancer using stable isotopes and high mass accuracyinstrumentation. J Proteome Res, 2006, 5: 1224-1231??
[18]  19 Koumenis I L, Vestal M L, Yergey A L, et al. Quantitation of metal isotope ratios by laser desorption time-of-flight mass spectrometry.Anal Chem, 1995, 67: 4557-4564??
[19]  20 Noga M J, Asperger A, Silberring J. N-terminal H3/D3-acetylation for improved high-throughput peptide sequencing by matrix-assisted laserdesorption/ionization mass spectrometry with a time-of-flight/time-of-flight analyzer. Rapid Commun Mass Spectrom, 2006, 20: 1823-1827??
[20]  21 Zappacosta F, Annan R S. N-terminal isotope tagging strategy for quantitative proteomics: results-driven analysis of protein abundancechanges. Anal Chem, 2004, 76: 6618-6627??
[21]  22 Burt A D. C L Oakley Lecture. Cellular and molecular aspects of hepatic fibrosis. J Pathol, 1993, 170: 105-114??
[22]  23 Azuma T, Koths K, Flanagan L, et al. Gelsolin in complex with phosphatidylinositol 4,5-bisphosphate inhibits caspase-3 and -9 to retardapoptotic progression. J Biol Chem, 2000, 275: 3761-3766
[23]  24 Neubauer K, Baruch Y, Lindhorst A, et al. Gelsolin gene expression is upregulated in damaged rat and human livers withinnon-parenchymal cells and not in hepatocytes. Histochem Cell Biol, 2003, 120: 265-275??
[24]  25 Suhler E, Lin W, Yin H L, et al. Decreased plasma gelsolin concentrations in acute liver failure, myocardial infarction, septic shock, andmyonecrosis. Crit Care Med, 1997, 25: 594-598??
[25]  26 Attallah A M, Zahran F, Ismail H, et al. Immunochemical identification and detection of serum fibronectin in liver fibrosis patients withchronic hepatitis C. J Immunoassay Immunochem, 2007, 28: 331-342??
[26]  27 Grieco A, Matera A, Di Rocco P, et al. Plasma levels of fibronectin in patients with chronic viral and alcoholic liver disease.Hepatogastroenterology, 1998, 45: 1731-1736
[27]  28 Perez Blanco F J, Rodriguez Cuartero A, Nunez Carril J, et al. Haptoglobin and hemopexin in the chronic hepatopathies. Rev Esp EnfermApar Dig, 1982, 62: 20-24
[28]  29 Poli V, Silengo L, Altruda F, et al. The analysis of the human hemopexin promoter defines a new class of liver-specific genes. NucleicAcids Res, 1989, 17: 9351-9365
[29]  30 Ratra R, Kar-Roy A, Lal S K. The ORF3 protein of hepatitis E virus interacts with hemopexin by means of its 26 amino acid N-terminalhydrophobic domain II. Biochemistry, 2008, 47: 1957-1969??
[30]  31 Koukoulis G K, Shen J, Virtanen I, et al. Vitronectin in the cirrhotic liver: an immunomarker of mature fibrosis. Hum Pathol, 2001, 32: 1356-1362??
[31]  32 Yamada S, Kobayashi J, Murawaki Y, et al. Collagen-binding activity of plasma vitronectin in chronic liver disease. Clin Chim Acta, 1996,252: 95-103??
[32]  33 Fan C, Stendahl U, Stjernberg N, et al. Association between orosomucoid types and cancer. Oncology, 1995, 52: 498-500??
[33]  34 Sorensson J, Matejka G L, Ohlson M, et al. Human endothelial cells produce orosomucoid, an important component of the capillary barrier.Am J Physiol, 1999, 276: H530-534
[34]  35 Lovat L B, Persey M R, Madhoo S, et al. The liver in systemic amyloidosis: insights from 123I serum amyloid P component scintigraphy in 484 patients. Gut, 1998, 42: 727-734??
[35]  36 Levo Y, Shalit M, Tur-Kaspa R. Serum amyloid P-component as a marker of liver disease. Am J Gastroenterol, 1982, 77: 427-430
[36]  37 Munthe-Fog L, Hummelshoj T, Honore C, et al. Immunodeficiency associated with FCN3 mutation and ficolin-3 deficiency. N Engl J Med,2009, 360: 2637-2644??
[37]  39 Giannelli G, Antonaci S. Immunological and molecular aspects of liver fibrosis in chronic hepatitis C virus infection. Histol Histopathol,2005, 20: 939-944
[38]  40 Mehta A S, Long R E, Comunale M A, et al. Increased levels of galactose-deficient anti-Gal immunoglobulin G in the sera of hepatitis Cvirus-infected individuals with fibrosis and cirrhosis. J Virol, 2008, 82: 1259-1270??
[39]  41 Ratziu V, Massard J, Charlotte F, et al. Diagnostic value of biochemical markers (FibroTest-FibroSURE) for the prediction of liver fibrosisin patients with non-alcoholic fatty liver disease. BMC Gastroenterol, 2006, 6: 6??
[40]  42 Gobel T, Vorderwulbecke S, Hauck K, et al. New multi protein patterns differentiate liver fibrosis stages and hepatocellular carcinoma inchronic hepatitis C serum samples. World J Gastroenterol, 2006, 12: 7604-7612
[41]  43 West R B, Harvell J, Linn S C, et al. Apo D in soft tissue tumors: a novel marker for dermatofibrosarcoma protuberans. Am J Surg Pathol,2004, 28: 1063-1069??
[42]  44 Otogawa K, Kinoshita K, Fujii H, et al. Erythrophagocytosis by liver macrophages (Kupffer cells) promotes oxidative stress, inflammation,and fibrosis in a rabbit model of steatohepatitis: implications for the pathogenesis of human nonalcoholic steatohepatitis. Am J Pathol, 2007,170: 967-980??
[43]  45 Poon T C, Hui A Y, Chan H L, et al. Prediction of liver fibrosis and cirrhosis in chronic hepatitis B infection by serum proteomicfingerprinting: a pilot study. Clin Chem, 2005, 51: 328-335??
[44]  46 Arteel G E. "HOPE" for the liver? Mechanistic insight into the role of the renin-angiotensin system in hepatic fibrosis. Hepatology, 2004, 40:263-265??
[45]  47 Xiao F, Wei H, Song S, et al. Polymorphisms in the promoter region of the angiotensinogen gene are associated with liver cirrhosis inpatients with chronic hepatitis B. J Gastroenterol Hepatol, 2006, 21: 1488-1491
[46]  48 Schiavon L L, Schiavon J L, Filho R J, et al. Simple blood tests as noninvasive markers of liver fibrosis in hemodialysis patients withchronic hepatitis C virus infection. Hepatology, 2007, 46: 307-314??
[47]  49 Mohamadnejad M, Montazeri G, Fazlollahi A, et al. Noninvasive markers of liver fibrosis and inflammation in chronic hepatitis B-virusrelated liver disease. Am J Gastroenterol, 2006, 101: 2537-2545
[48]  50 Lebensztejn D M, Skiba E, Sobaniec-Lotowska M, et al. A simple noninvasive index (APRI) predicts advanced liver fibrosis in childrenwith chronic hepatitis B. Hepatology, 2005, 41: 1434-1435??
[49]  51 Murata C, Watanabe T, Furuya H, et al. Aldehyde dehydrogenase 2 and beta3-adrenergic receptor gene polymorphisms: their associationwith elevated liver enzymes and metabolic syndrome. Metabolism, 2003, 52: 1096-1101??
[50]  52 Enomoto N, Takase S, Takada N, et al. Alcoholic liver disease in heterozygotes of mutant and normal aldehyde dehydrogenase-2 genes.Hepatology, 1991, 13: 1071-1075??
[51]  53 Ferre N, Marsillach J, Camps J, et al. Genetic association of paraoxonase-1 polymorphisms and chronic hepatitis C virus infection. ClinChim Acta, 2005, 361: 206-210
[52]  54 Shirai R, Hirano F, Ohkura N, et al. Up-regulation of the expression of leucine-rich alpha(2)-glycoprotein in hepatocytes by the mediators ofacute-phase response. Biochem Biophys Res Commun, 2009, 382: 776-779??
[53]  55 Heo S H, Lee S J, Ryoo H M, et al. Identification of putative serum glycoprotein biomarkers for human lung adenocarcinoma by multilectinaffinity chromatography and LC-MS/MS. Proteomics, 2007, 7: 4292-4302??
[54]  56 De Sa S V, Correa-Giannella M L, Machado M C, et al. Serpin peptidase inhibitor clade A member 1 as a potential marker for malignancyin insulinomas. Clin Cancer Res, 2007, 13: 5322-5330??
[55]  57 Sathe S, Sakata M, Beaton A R, et al. Identification, origins and the diurnal role of the principal serine protease inhibitors in human tearfluid. Curr Eye Res, 1998, 17: 348-362??
[56]  58 蒋洪彦, 王世鑫, 李雪华, 等. 严重急性呼吸综合征康复人员并发股骨头缺血性坏死血清相关蛋白研究. 中华预防医学杂志, 2008,42: 522-526
[57]  59 Zhou M, Shen D, Head J E, et al. Ocular clusterin expression in von Hippel-Lindau disease. Mol Vis, 2007, 13: 2129-2136
[58]  60 Rodriguez-Pineiro A M, de la Cadena M P, Lopez-Saco A, et al. Differential expression of serum clusterin isoforms in colorectal cancer.Mol Cell Proteomics, 2006, 5: 1647-1657?? 61 Chen X, Halberg R B, Ehrhardt W M, et al. Clusterin as a biomarker in murine and human intestinal neoplasia. Proc Natl Acad Sci USA,2003, 100: 9530-9535??
[59]  62 Kang Y K, Hong S W, Lee H, et al. Overexpression of clusterin in human hepatocellular carcinoma. Hum Pathol, 2004, 35: 1340-1346??
[60]  63 Meistermann H, Norris J L, Aerni H R, et al. Biomarker discovery by imaging mass spectrometry: transthyretin is a biomarker forgentamicin-induced nephrotoxicity in rat. Mol Cell Proteomics, 2006, 5: 1876-1886??
[61]  64 Biroccio A, Del Boccio P, Panella M, et al. Differential post-translational modifications of transthyretin in Alzheimer''s disease: a study ofthe cerebral spinal fluid. Proteomics, 2006, 6: 2305-2313??
[62]  65 Barcelo-Batllori S, Kalko S G, Esteban Y, et al. Integration of DIGE and bioinformatics analyses reveals a role of the antiobesity agenttungstate in redox and energy homeostasis pathways in brown adipose tissue. Mol Cell Proteomics, 2008, 7: 378-393
[63]  66 Saile B, Ramadori G. Inflammation, damage repair and liver fibrosis-- role of cytokines and different cell types. Z Gastroenterol, 2007, 45: 77-86??
[64]  67 Kershenobich S D, Weissbrod A B. Liver fibrosis and inflammation: a review. Ann Hepatol, 2003, 2: 159-163
[65]  38 Le Y, Lee S H, Kon O L, et al. Human L-ficolin: plasma levels, sugar specificity, and assignment of its lectin activity to the fibrinogen-like(FBG) domain. FEBS Lett, 1998, 425: 367-370??
[66]  68 Yip T T, Cho W C, Cheng W W, et al. Application of ProteinChip array profiling in serum biomarker discovery for patients suffering fromsevere acute respiratory syndrome. Methods Mol Biol, 2007, 382: 313-331??
[67]  69 Spisak S, Tulassay Z, Molnar B, et al. Protein microchips in biomedicine and biomarker discovery. Electrophoresis, 2007, 28: 4261-4273??
[68]  70 Zhang G, Terry A V Jr, Bartlett M G. Liquid chromatography/tandem mass spectrometry method for the simultaneous determination of olanzapine,risperidone, 9-hydroxyrisperidone, clozapine, haloperidol and ziprasidone in rat plasma. Rapid Commun Mass Spectrom, 2007, 21: 920-928
[69]  71 Boersema P J, Raijmakers R, Lemeer S, et al. Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics. Nat Protoc,2009, 4: 484-494??
[70]  1 Lee W M. Hepatitis B virus infection. N Engl J Med, 1997, 337: 1733-1745??

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133