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基于抗体的水稻蛋白质组学——开端与展望

DOI: 10.1360/052010-760, PP. 173-177

Keywords: 基于抗体的水稻蛋白质组学,水稻,抗体,免疫印迹

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Abstract:

为了深入开展水稻基因功能研究,借鉴人类蛋白质研究中的相关理念,提出了基于抗体的水稻蛋白质组学的策略.AbRP的要点在于,建立针对水稻蛋白质的抗体资源库,利用大规模免疫分析技术,揭示水稻蛋白质在不同时空条件下的表达状态并了解其相关功能.用AbRP策略可以对特定的基因家族或途径相关蛋白质进行研究,可以检测蛋白质的表达量及修饰的变化等.AbRP的实施将为水稻基础和应用研究创建一个重要的支撑平台,基于抗体的蛋白质表达数据与基因组、转录组数据相整合,可望进一步丰富水稻乃至其他植物研究的公共数据资源.

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