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稻瘟病抗病基因Pia的抗性分析及精细定位

, PP. 70-77

Keywords: 稻瘟病,抗病基因,Pia,精细定位,基因型

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Abstract:

稻瘟病是水稻最主要的病害之一.持久抗性品种可以通过聚合不同类型的抗病基因而获得.利用中国10个地区的稻瘟病群体对日本鉴别品种(爱知旭)所持的抗病基因Pia进行了抗谱分析.该基因除了在江苏群体中表现为强效抗性之外,在其他群体中均表现为弱效抗性.利用爱知旭/Kasalath的F2作图群体,通过重组体筛选、精细定位以及共分离分析,将Pia位点界定于约90kb的物理区段内.利用日本晴的参考序列,在该区段内预测出具有抗病基因保守结构的4个侯选基因(Pia-1~Pia-4);相应开发出4个CRG(candidateresistancegene)标记(A17,A25,A26和A27)并进行了共分离分析.结果表明,前3个标记都与Pia位点完全共分离,而来源于Pia-4的A27为抗病亲本缺失的显性标记,且该标记位点与Pia及其侧翼标记位点不连锁.由此推断,包含Pia-4的区域并不在Pia位点,因而可以将其排除.利用上述3个共分离的标记,对日本、中国和IRRI(国际水稻所)开发的3套鉴别品种进行了抗病基因型与分子标记基因型的相关分析.结果表明,Pia-3为最可能的候选基因.本研究为下一步克隆该基因并了解其在稻瘟病抗性中的基础作用提供了依据.

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