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流感病毒唾液酸受体在人、小鼠、鸡及鸭气管和肺组织分布的定性与定量分析

DOI: 10.1360/052012-76, PP. 383-389

Keywords: 流感病毒,唾液酸受体,气管,,凝集素,免疫荧光,定量分析,定性分析

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Abstract:

为了准确地检测流感病毒唾液酸α2,3-半乳糖和唾液酸α2,6-半乳糖两种受体在人、小鼠、鸡及鸭气管和肺组织中分布和表达的特征,本研究利用凝集素免疫荧光组织染色方法对各种组织切片进行了系统的定性和定量分析.定性分析的结果,显示两种流感病毒唾液酸受体在人、小鼠、鸡及鸭肺组织和小鼠及鸭气管组织中均有表达,但是,唾液酸α2,6-半乳糖受体只在人气管组织中有表达而唾液酸α2,3-半乳糖受体只在鸡气管组织中有表达.定量分析的结果显示,两种流感病毒唾液酸受体在人和小鼠的气管组织以及人、鸡和鸭的肺组织均有表达.同时,本研究结果还显示了流感病毒受体在同一组织中的分布和表达并不是均匀的,表明它们在各种组织中的分布和表达并非简单的有和无之间的区别,而是一种在表达数量上多和少的差别.

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