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小鼠冷冻精子的胚胎发育和表观遗传学

, PP. 374-382

Keywords: 精子冷冻,单精子卵胞浆内注射,表观遗传学,组蛋白修饰,DNA甲基化

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Abstract:

精子冷冻是保存具有生物医学研究价值的珍稀动物种群的一种重要手段.随着卵胞浆内单精子注射技术的广泛应用,寻求更加简便的精子保存方法显得尤为重要.配子在体外培养时的环境和操作都可能对其表观遗传的重组产生影响.通过ICSI产生胚胎,本研究对-20℃保存的不添加冷冻保护剂的精子检测了包括DNA甲基化和组蛋白修饰在内的胚胎表观遗传重组状态.结果显示,尽管冷冻精子的受精能力以及胚胎发育率有所下降,但与新鲜精子组相比,其胚胎的表观遗传重组动态进程并没有改变.以上结果提示,精子在-20℃冻存不改变胚胎的表观遗传.

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