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DNA重组酶同系物stRadC2在超嗜热古菌Sulfolobustokodaii细胞内的功能分析

, PP. 226-233

Keywords: 古菌,硫化叶菌,重组,RadA,stRadC,Hjc

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Abstract:

真核生物和广古菌的Rad51/RadA同系物在重组和修复过程中起重要作用,比如Rad51同系物Rad51C的突变体与人类乳腺癌和卵巢癌的发生直接相关.超嗜热广古菌硫化叶菌(Sulfolobustokodaii)编码4个RadA同系物,它们序列上与RadA相近,但在细胞内的功能还不清楚.对这些蛋白的研究有助于更好地了解人类Rad51同系物的功能.本文对其中一个RadA同系物stRadC2蛋白的功能进行了研究.Pull-down分析发现,stRadC2能够直接和DNA重组酶RadA和Hollidayjunction内切酶Hjc相互作用.进一步的酶学分析证明,stRadC2能够抑制RadA的链交换活性并促进Hjc对HollidayjunctionDNA的切割活性.RT-PCR分析发现,虽然stRadC2的表达不能被紫外线(UV)辐射诱导,但是它可在DNA修复后期迅速恢复表达,上述结果表明,stRadC2蛋白可能作为一种抗重组酶,在DNA重组修复的后期发挥作用.

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