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生物活性玻璃-胶原-磷酸丝氨酸复合支架的调控矿化和生物反应

, PP. 240-245

Keywords: 复合,支架,细胞,生物矿化,调控

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Abstract:

应用组织工程的策略制备仿生复合支架是修复骨组织缺损的一种新兴方法.本研究通过混合交联生物活性玻璃、胶原和磷酸丝氨酸,制备了一种新型的仿生复合支架.该支架具有与天然骨组织相类似的成分.体外实验表明,复合支架在不含MC3T3-E1细胞的培养液中矿化产生了八面体晶体,与复合支架和细胞共同培养过程中的矿化产物在形态和成分上一致.这种八面体晶体最后演变成板状羟基磷灰石.支架的细胞外基质类似组分对矿化具有明显的调控作用.体内动物实验证实,该复合支架具有优良的骨修复性能.

References

[1]  1 Dalby M J, Childs S, Riehle M O. Fibroblast reaction to island topography: changes in cytoskleton and morphology with time. Biomaterials,2003, 24: 927–935??
[2]  2 Palmer L C, Newcomb C J, Kaltz S R. Biomimetic systems for hydroapatite mineralization inspired by bone and enamel. Chem Rev, 2008,108: 4754–4783??
[3]  3 Dalby M J, Riehle M O, Johnstone H. In vitro reaction of endothelial cells to polymer demixed nanotopography. Biomaterials, 2002, 23:2945–2954??
[4]  4 Barbucci R, Magnani A, Lamponi S. The use of hyaluronan and its sulphated derivative patterned with micrometric scale on glass substrate in melanocyte cell behaviour. Biomaterials, 2003, 24: 915–926??
[5]  5 王桢, 张正, 张俊川, 等. 体积较大的多孔聚酯支架中的骨髓基质干细胞的分布. 科学通报, 2009, 54: 1207–1213
[6]  6 吴涛, 南开辉, 陈景帝, 等. 可控释淫羊藿苷的仿生组装骨诱导修复材料. 科学通报, 2009, 54: 1198–1206
[7]  7 Silver I A, Deas J, Erecinska M. Interactions of bioactive glasses with osteoblasts in vitro: effects of 45S5 Bioglass, and 58S and 77S bioactive glasses on metabolism, intracellular ion concentrations and cell viability. Biomaterials, 2001, 22: 175–185
[8]  8 张举成, 刘卫, 易中周, 等. 竹红菌乙素光动力作用调控胶原蛋白中吡啶啉交联含量: 光动力治疗纤维化症机制的初探. 科学通报,2009, 54: 2076–2081
[9]  9 Xynos I D, Hukkanen M V J, Batten J J. Gene expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. Calcif Tissue Int, 2000, 67: 321–329??
[10]  10 Varma H K, Yokogawa Y, Espinosa F F, et al. Porous calcium phosphate coating over phosphorylated chitosan film by a biomimetic method. Biomaterials, 1999, 20: 879–884??
[11]  11 Cui F Z, Wang Y, Zhang W. Conformation change of collagen during the initial stage of biomineralization of calcium phosphate. J Mater Sci-Mater, 2008, 18: 35–38
[12]  12 Hench L L. Bioceramics. J Am Ceram Soc, 1998, 81: 1705–1728
[13]  13 Place E S, Evans N D, Stevens M M. Complexity in biomaterials for tissue engineering. Nat Mater, 2009, 8: 457–470??
[14]  14 Rafat M, Li F, Fagerholm P. PEG-stabilized carbodiimide crosslinked collagen- chitosan hydrogels for corneal tissue engineering. Biomaterials, 2008, 29: 3960–3972??
[15]  15 Cao H, Xu S Y. EDC/NHS-crosslinked type II collagen-chondroitin sulfate scaffold: characterization and in vitro evaluation. J Mater Sci Mater Med, 2008, 19: 567–575??
[16]  16 Wang Y J, Yang C R, Chen X F. Investigation of the porous biomaterials for bone reconstruction with addition of biomimetic nano-sized inorganic particles. Key Eng Mater, 2007, 336–338: 1534–1537??

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