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基于四引物扩增受阻突变体系PCR的多重乳腺癌相关SNP位点检测方法的建立

DOI: 10.1360/052011-628, PP. 143-147

Keywords: 单核苷酸多态分型,四引物扩增受阻突变体系PCR,多重PCR,毛细管电泳

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Abstract:

为提高单核苷酸多态性检测的通量,引入多重嵌合引物PCR和毛细管电泳对四引物扩增受阻突变体系PCR进行改进.针对乳腺癌位点rs4784227(C>T),rs1219648(G>A)和rs3803662(T>C)设计特异性嵌合引物,经一次PCR扩增后,通过毛细管电泳分析产物长度,同时确定3个位点的基因型.70份全血和口腔拭子样本,电泳结果均与测序一致,实现成功分型.本方法仅需一次PCR和一次毛细管电泳即可获得3个位点的分型结果,操作简单、快速准确.

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