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线粒体稳定高钙信号诱发超氧炫

DOI: 10.1360/052013-281, PP. 869-876

Keywords: 超氧炫,钙信号,活性氧,线粒体

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Abstract:

线粒体对于细胞钙信号和活性氧信号转导有重要的调控作用.超氧炫是新近发现的单个线粒体超氧阴离子短时程爆发现象,反映了活性氧生成动力学的一种新形式.线粒体钙信号作为重要的细胞功能调控信号,能否及如何调控超氧炫尚待深入研究.本研究对HeLa细胞进行高胞外钙和离子霉素刺激,或用皂苷穿孔细胞质膜后置于高钙细胞内液中,两种方法均显著增加了超氧炫发生的频率.其中,穿孔细胞胞浆高钙诱导的超氧炫依赖于线粒体钙单向转运体,表明超氧炫由线粒体基质内高钙信号所诱发.重要的是,离子霉素诱导的超氧炫发生频率与线粒体稳态钙水平线性相关,而与瞬态线粒体钙无相关性,提示钙离子对超氧炫的调控是一个多步骤、相对缓慢的过程.综上,线粒体基质的稳态高钙是超氧炫的重要调控因子.

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