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细胞周期因子geminin对血管平滑肌细胞增殖是不必要的

, PP. 745-752

Keywords: geminin,DNA复制,细胞周期,增殖,血管平滑肌细胞

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Abstract:

血管平滑肌细胞增殖是心血管疾病重要的病理生理机制.geminin能够通过调控DNA复制和细胞周期进程在癌细胞的增殖中发挥重要的作用.因此,本研究假设geminin调控了血管平滑肌细胞的增殖.研究结果显示,在静止的血管平滑肌细胞中(约90%细胞处于G1期,10%细胞处于S,G2/M期)geminin低表达,随着更多的细胞进入S,G2/M期,geminin表达水平逐渐升高,而当细胞退出S,G2/M期,geminin表达水平逐渐下降.心血管病理因子血管紧张素Ⅱ和去甲肾上腺素也能刺激血管平滑肌细胞高表达geminin.然而,进一步在血管平滑肌细胞中敲低和过表达geminin后,敲低组和过表达组展示出与各自对照组相似的DNA含量、细胞核形态、细胞周期比例及细胞增殖能力.这一研究结果显示,geminin对血管平滑肌细胞的增殖并不是必需的,这与既往在癌细胞系中的研究结果是不同的,geminin在正常细胞和癌细胞系的这种功能差异提示其可作为一个肿瘤治疗的靶点.

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